Breaking News
November 19, 2017 - Dental filling failure linked to smoking, drinking and genetics
November 19, 2017 - Medicaid expansion takes a bite out of medical debt
November 19, 2017 - Study aims to involve parents in early intervention services for children with autism
November 19, 2017 - Don’t Fret Delays in Treating Colon Cancer, Study Suggests
November 19, 2017 - Yeast Infection Test: MedlinePlus Lab Test Information
November 19, 2017 - Video of blood clot contraction reveals how platelets naturally form unobtrusive clots
November 19, 2017 - UNN researchers explore modeling of cognitive dissonance phenomenon
November 19, 2017 - Verseon presents second anticoagulation candidate for clinical trials at AHA 2017
November 19, 2017 - Pregnant Asian women who develop high blood pressure at highest risk for heart failure hospitalization
November 19, 2017 - Medicare seeks comment on ways to cut costs of Part D drugs
November 19, 2017 - Tree cover linked to fewer asthma cases in polluted urban neighborhoods
November 19, 2017 - Make the Diagnosis: Hair Care Conundrum
November 19, 2017 - New techniques give blood biopsies greater promise
November 19, 2017 - Researchers identify possible genetic basis for coronary artery disease
November 19, 2017 - Stress experienced by emergency call handlers has negative impact on psychological health
November 18, 2017 - New cancer cell screening is improving childhood leukaemia treatment
November 18, 2017 - Groundbreaking study identifies protein as potential factor in cancer metastasis
November 18, 2017 - New model to test effectiveness of existing and potential CF therapies
November 18, 2017 - Staying Active May Lower Odds for Glaucoma
November 18, 2017 - Potential new autism drug shows promise in mice
November 18, 2017 - Some states roll back ‘retroactive Medicaid,’ a buffer for the poor — and for hospitals
November 18, 2017 - Selectively deleting stem cell factor promotes recovery after TBI in mice
November 18, 2017 - Breakthrough research brings new procedure closer to helping patients with blood cancer
November 18, 2017 - Dr Peter Simpson Appointed to SLAS Board of Directors
November 18, 2017 - Friendships between young children can protect against ADHD
November 18, 2017 - Old World monkeys could hold key to stop progression of rheumatoid arthritis
November 18, 2017 - Harris Health System RNs named among 20 Outstanding Nurses for 2017
November 18, 2017 - Old World monkeys could be key to a new, powerful rheumatoid arthritis therapy
November 18, 2017 - Mount Sinai researchers identify new therapeutic target for ER+ breast cancer
November 18, 2017 - Age, CRP levels predict success in tapering of biologics in rheumatoid arthritis patients
November 18, 2017 - New dye could be used to observe electrical activity of neurons in the brain
November 18, 2017 - New study further validates use of vaginal progesterone to decrease risk of preterm birth
November 18, 2017 - Russian researcher determined range of reference values for boron in the human body
November 18, 2017 - ‘What the Health?’ Tax bill or health bill?
November 18, 2017 - Could Your Cat Give You ‘Bird Flu?’
November 18, 2017 - Vitamin D Linked to Fertility Outcomes in ART
November 18, 2017 - Neuroscientists identify genetic changes in microglia in a mouse model of neurodegeneration and Alzheimer’s disease
November 18, 2017 - Tax reform proposal could impact care for older Americans
November 18, 2017 - PCSK9 inhibitor offers clinical benefit to patients with peripheral artery disease
November 18, 2017 - Researchers receive £1.3 million to develop sight-saving imaging technology
November 18, 2017 - Novel buckypaper sensor could pave way for high-performance, affordable wearable technology
November 18, 2017 - Despite ACA cost protections, most adolescents skip regular checkups
November 18, 2017 - Stem cell treatment allows paraplegic rats to walk and regain sensory perception
November 18, 2017 - HTC analytical conference comes to the UK
November 18, 2017 - Face It: Drinking, Smoking Takes Toll on Looks: MedlinePlus Health News
November 18, 2017 - New research shows where in the brain the earliest signs of Alzheimer’s occur
November 18, 2017 - Philips announces launch of global movement to raise awareness for COPD
November 18, 2017 - University of Bristol awarded grant to reduce antibacterial drug resistance in Thailand
November 18, 2017 - New oxytocin chemical sensor could be first step towards early diagnosis of autism
November 18, 2017 - Study shows how naïve T-cells may affect tumor immunity and immunotherapy
November 18, 2017 - New studies highlight importance of cardiorespiratory fitness to reduce CVD risk
November 18, 2017 - Study finds medical cannabis is effective at reducing opioid addiction
November 18, 2017 - Specially tailored, ultrafast light pulses can trigger neurons to fire in different patterns
November 18, 2017 - Decrease in sunshine linked to rising incidence of Rickets
November 18, 2017 - Harnessing social media big data to fight against prescription drug crisis
November 18, 2017 - Researchers find way to switch tumor cells between 2D and 3D morphology
November 18, 2017 - Leaf-eating ability of beetle largely due to bacteria inside the insect
November 18, 2017 - FDA Approves Hemlibra (emicizumab-kxwh) for Hemophilia A with Inhibitors
November 18, 2017 - Adolescents underreport amphetamine use, likely unaware that adderall is amphetamine
November 18, 2017 - Study reveals a reduced risk of teenage eczema in breastfed babies
November 18, 2017 - Separating side effects could pave way for safe, effective pain medications
November 18, 2017 - Gut bacteria at young age can contribute to MS disease onset and progression, study suggests
November 18, 2017 - Environmental triggers may play role in development of Lupus
November 18, 2017 - Review looks into conventional versus new treatment modalities in orthodontic pain management
November 17, 2017 - FDA Alert: Diphenoxylate Hydrochloride and Atropine Sulfate Tablets by Greenstone: Recall
November 17, 2017 - For older women, every movement matters
November 17, 2017 - Talking-based therapy could transform aftercare for cancer survivors
November 17, 2017 - Olympus IXplore SpinSR10 imaging system enables researchers to observe fine details in live cells
November 17, 2017 - Study explores reasons for underrepresentation of minorities in genetic cancer research
November 17, 2017 - California firm running physician practices is closing down as scrutiny ramps up
November 17, 2017 - BMI not valid measure of obesity in postmenopausal women, study shows
November 17, 2017 - Vaginal progesterone decreases the risk of premature birth in women with short cervix
November 17, 2017 - Pricey ER Tests for Chest Pain Often Unnecessary
November 17, 2017 - ‘Old’ Lungs May Be Good Transplant Options: MedlinePlus Health News
November 17, 2017 - How not to gain weight over the holidays
November 17, 2017 - Researchers map first-ever proteome of healthy human heart
November 17, 2017 - Drug used to prevent and treat malaria may also be effective for Zika virus
November 17, 2017 - One in 20 children still receiving codeine to treat pain despite warning from federal regulators
November 17, 2017 - Improving clinical trials with machine learning
November 17, 2017 - Experts identify mental exercise program that can reduce risk of dementia
Gene enhancing method is helping discover attainable new drug goals for KRAS-driven cancers

Gene enhancing method is helping discover attainable new drug goals for KRAS-driven cancers

image_pdfDownload PDFimage_print

Cancers pushed by means of mutations within the KRAS gene are a few of the most threatening. For many years, researchers have attempted unsuccessfully to without delay goal mutant KRAS proteins as a method to regard tumors. As an alternative of focused on mutant KRAS itself, researchers at College of California San Diego College of Drugs are actually in search of different genes or molecules that, when inhibited, kill most cancers cells best when KRAS could also be mutated.

The workforce used the CRISPR-Cas9 gene enhancing method to systematically inactivate each gene within the genome of human colorectal most cancers cells with and with out mutant KRAS. They discovered that expansion of KRAS-mutant colorectal most cancers cells in mice used to be lowered by means of roughly 50 % when two genes that encode metabolic enzymes — NADK and KHK — have been additionally inactivated.

The learn about, revealed September 27 in Most cancers Analysis, supplies attainable new drug goals for KRAS-driven cancers.

“We didn’t get those identical effects with most cancers cells grown within the lab — the expansion inhibition we noticed when the NADK and KHK genes have been inactivated best happens in tumors in a mammalian gadget, in a extra real looking microenvironment the place the tumor has to live to tell the tale,” mentioned senior writer Tariq Rana, PhD, professor of pediatrics at UC San Diego College of Drugs and Moores Most cancers Heart. “That means that the metabolic dependencies of tumor cells rising in a laboratory dish might fluctuate dramatically in comparison to the similar cells rising in a dwelling gadget, underscoring attainable boundaries of usual laboratory-based most cancers mobile expansion exams.”

Roughly 20 to 30 % of all human cancers have mutations within the KRAS gene. KRAS mutations happen in most of the maximum deadly and maximum tough to regard cancers, together with lung, pancreatic and colorectal most cancers. KRAS mutant most cancers cells are ready to rewire their metabolism in some way that provides them a expansion benefit in comparison to customary cells.

Rana’s option to treating KRAS-driven cancers — inhibiting different genes or molecules along with KRAS — is named “artificial lethality” since the intervention is best deadly to the mutated cells. In a prior learn about, Rana’s workforce used a library of microRNAs, small items of genetic subject matter, to systematically block protein manufacturing and search for the ones inhibitions which can be artificial deadly together with KRAS mutations.

Of their newest learn about, Rana’s workforce used CRISPR-Cas9 to systematically inactivate genes in two human colorectal most cancers mobile traces — one with customary KRAS and one with a mutant KRAS. They then examined the power of each and every of those mobile traces to develop as tumors in mice. They discovered that inactivating two metabolic enzymes, NADK and KHK, lowered the expansion of KRAS-mutant tumors by means of roughly 50 %, however had no impact on customary KRAS tumors. Additionally they blocked those identical enzymes with commercially to be had small molecule inhibitors and noticed vital relief in tumor expansion in mice best in tumor cells with mutant KRAS.

Rana and workforce additionally known a number of new genes that, when inactivated, had the other impact — they higher KRAS-mutant tumor expansion, however now not the expansion of ordinary KRAS tumors. All these genes are referred to as “tumor suppressors” as a result of they typically stay most cancers mobile expansion in test.

“Some of the sudden findings from our learn about is how acting this sort of genetic display screen without delay in a mammalian microenvironment published now not best new artificial deadly interactions, but additionally new tumor suppressor genes which can be depending on KRAS mutations,” mentioned first writer Edwin Yau, MD, PhD, a hematology/oncology and Most cancers Therapeutics Coaching Program fellow in Rana’s lab.

The sort of new tumor suppressor genes encodes INO80C, a big multi-subunit protein that, amongst different issues, stabilizes the genome. Rana, Yau and co-workers are actually taking steps to hold their findings ahead, with without equal objective of higher working out how KRAS-mutant cancers expand and translating those insights into creating new remedies to prevent them.

Supply:

https://well being.u.s.a..edu/information/releases/Pages/2017-09-27-editing-genes-throughout-colorectal-cancer-cell-genome-uncovers-drug-target.aspx

Tagged with:

About author

Related Articles