Breaking News
November 21, 2017 - Simple test could help predict best treatment for cystic fibrosis patients
November 21, 2017 - Diabetes patients’ immune system can regulate insulin production
November 21, 2017 - Simple mirror technique boosts the performance of diSPIM, study says
November 21, 2017 - FDA raids Florida stores that consumers use to buy drugs from Canada
November 21, 2017 - FDA Alert: Limbrel Capsules by Primus Pharmaceuticals: FDA Advisory
November 21, 2017 - Exploring the genetics of glaucoma and retinal development
November 21, 2017 - Study finds sensors to detect biomarkers
November 21, 2017 - Researchers develop simple test to predict T2D remission after bariatric surgery
November 21, 2017 - Penn researchers find evidence of new malaria species in wild bonobos
November 21, 2017 - Review examines effectiveness of lipid-based products to treat dry eye disease
November 21, 2017 - Pioneering research project aims to develop better diagnostic tests for NAFLD
November 21, 2017 - The Best and Worst Ways to Say ‘I Love You’
November 21, 2017 - Even Partial Breast-Feeding for First Few Months Lowers SIDS Risk: MedlinePlus Health News
November 21, 2017 - Researchers identify protein that plays key role in diabetic blindness
November 21, 2017 - Sensors for identifying biomarkers could help detect myriad of diseases at early stages
November 21, 2017 - ‘Chemo brain’ may start as tumors form and develop, suggests study
November 21, 2017 - Scientists develop new method to study Creutzfeldt-Jakob disease in the lab
November 21, 2017 - Researchers identify previously unknown breast cell types
November 21, 2017 - Probing living cells with AFM
November 21, 2017 - Streamlined method for using CGM helps patients with diabetes maintain safe, stable glucose levels
November 21, 2017 - Even Advanced Breast Cancer Patients Gain From Exercise: MedlinePlus Health News
November 21, 2017 - Study in mice finds dietary levels of genistein may adversely affect female fertility
November 21, 2017 - Disordered eating has detrimental effects to long-term health of young adults, study finds
November 21, 2017 - Babies know more than we think they do find researchers
November 21, 2017 - New technique may reveal clues about role of centromeres in Down syndrome, other birth defects
November 21, 2017 - IUD May Lower Cervical Cancer Risk: MedlinePlus Health News
November 21, 2017 - U.S. preemie birth rates rise two years in a row
November 21, 2017 - Heart rate variability measured by electrocardiogram can help differentiate bipolar from major depression
November 21, 2017 - $10 million gift creates new center at Northwestern University to study effects of environment on genes
November 21, 2017 - Uncontrolled lung diseases could lead to costly A&E visits and hospital stays
November 21, 2017 - New study explores patients’ and family members’ views on overlapping surgeries
November 21, 2017 - KNAUER celebrates 55th anniversary and awards science prize
November 21, 2017 - Researchers find new way to boost cancer-destroying ability of T-cells
November 21, 2017 - Maintaining adequate vitamin D may help prevent onset of inflammatory diseases
November 21, 2017 - What NPs Can Do in the Opioid Crisis
November 21, 2017 - Gun Injuries Getting More Severe, Experts Say: MedlinePlus Health News
November 21, 2017 - That music playing in your head is a real conundrum for scientists
November 21, 2017 - Researcher awarded $1.5 million to study role of tumor necrosis factor in IBD
November 21, 2017 - Digital pills can help track opioid use patterns
November 21, 2017 - UZH leads Europe’s largest research on aging
November 21, 2017 - Experts endorse recommendations for restrictive blood transfusions
November 21, 2017 - New Hemophilia A Treatment, Hemlibra (Emicizumab-kxwh), Stems Bleeding Episodes
November 21, 2017 - Shining a light on the nervous system to thwart disease
November 21, 2017 - Use of Prostate Health Index cuts down need for uncomfortable biopsies
November 21, 2017 - Drugstore pain pills as effective as opioids in ER patients
November 21, 2017 - Experts explain why lead found in fidget spinners is no idle threat
November 21, 2017 - Over-the-counter decongestant found to be effective inhibitor of tumor stroma
November 21, 2017 - Anticholinergic Cognitive Burden Scale shows dose-response relationship with adverse outcomes
November 21, 2017 - Non-vitamin K antagonist oral anticoagulants linked to reduced kidney risks
November 21, 2017 - New technique holds promise to screen cancer cells for drug susceptibility
November 21, 2017 - AstraZeneca wins US approval for lymphoma drug
November 21, 2017 - Mother’s diet can protect nursing newborns against food allergies, research shows
November 21, 2017 - Cystic fibrosis patients have more good and bad bacteria in their lungs, study finds
November 21, 2017 - Patient-centered medical home model effective at improving chronic disease outcomes
November 21, 2017 - Enterovirus vaccine protects against virus-induced diabetes in mouse model
November 20, 2017 - First-of-its-kind study combines smoking cessation with personalized medicine
November 20, 2017 - FDA Approves Sutent (sunitinib malate) for Adjuvant Treatment of Adult Patients at High Risk of Recurrent Renal Cell Carcinoma
November 20, 2017 - Researchers produce the first draft cell atlas of the small intestine
November 20, 2017 - Study explores feasibility of mainstreaming genetic counseling for ovarian cancer
November 20, 2017 - AAP and The Obesity Society jointly address weight stigma in childhood obesity
November 20, 2017 - Researchers identify three specific molecules that accurately indicate pre-diabetes
November 20, 2017 - Study estimates health care costs, injuries, and deaths due to motorcycle accidents
November 20, 2017 - Face It: Drinking, Smoking Takes Toll on Looks
November 20, 2017 - Women have lower tolerance to alcohol following gastric sleeve surgery, study finds
November 20, 2017 - Salt, inflammation and hypertension
November 20, 2017 - Electrochemistry method provides easy access to important classes of compounds
November 20, 2017 - Cancer drugs may offer new way to control high blood pressure
November 20, 2017 - Small molecules in saliva may offer clues to diagnose and predict duration of concussions
November 20, 2017 - New poll demonstrates challenge of finding childcare options that meet parent’s safety criteria
November 20, 2017 - Canadians root for an underdog U.S. health policy idea
November 20, 2017 - KAIST researchers identify principles of gene network for colon tumorigenesis
November 20, 2017 - Pregnant mother in a coma wakes three months after delivery
November 20, 2017 - VTT researcher develops disposable optical test substrate for microbial detection
November 20, 2017 - Benzodiazepines linked to increased risk of death among Alzheimer’s disease patients
November 20, 2017 - Women are advised that sleeping on their side could reduce stillbirth risk
November 20, 2017 - The skinny on lipid immunology
November 20, 2017 - Study sheds light on changes in young people’s sexual practices
November 20, 2017 - Study highlights need for early identification, treatment of PTSD in cancer survivors
November 20, 2017 - FDA approves remote programming feature for Nucleus Cochlear Implant System via telemedicine
November 20, 2017 - The One Patient That We Neglect Too Often
Researchers find efficient way to produce neurons from pluripotent stem cells

Researchers find efficient way to produce neurons from pluripotent stem cells

image_pdfDownload PDFimage_print

Since their discovery in 2006, induced pluripotent stem cells are a glimmer of hope for many diseases. But further research of the complex regulation of pluripotent stem cell identity revealed unexpected difficulties. A team of researchers at the Cluster of Excellence CECAD has now found an efficient way to produce neurons from pluripotent stem cells. Their research was published in Nature Communications.

As the origin of multicellular organisms, pluripotent stem cells can differentiate into all the cell types of the body. These cells can replicate indefinitely in culture and, therefore, are considered immortal. The gold standard of pluripotency is the embryonic stem cell (ESC). Somatic cells such as skin cells can be reprogrammed to generate induced pluripotent stem cells (iPSCs) that share similar characteristics with ESCs. As such, pluripotent stem cells hold a great promise for regenerative medicine as a potential source of healthy differentiated cells, including neurons. Moreover, these cells represent an invaluable resource to investigate human development and disease in the relevant cells (neurons) affected in disorders such as Alzheimer´s, Huntington’s or Parkinson´s.

Neuronal differentiation protocols of pluripotent stem cells are usually expensive and generate a mixture of different neuronal cells and other cell types. By knocking down a single gene, the team led by David Vilchez, was able to produce neurons with 100% efficiency: “By silencing one single protein with the gene-editing method CRISPR, the cells spontaneously start to differentiate into neurons! That´s a great and much faster way to increase neurogenesis.” In natural conditions, this factor called CSDE1 prevents differentiation and keeps the cells in a pluripotent state. “This could be a very powerful mechanism to have pure populations of neurons and to facilitate a better understanding of neurodegenerative diseases.”

Hyun Ju Lee, first author of the study was most excited about the fast changes observed in her assays: “We could visualize the changes and really see it happening, the differentiation goes really fast. We also double-checked in multiple stem cell lines from different donors and induced pluripotent stem cells and got the same results.” For the study, human embryonic stem cells, induced pluripotent stem cells and mouse stem cells were used.

By using the new approach, it would be possible to facilitate the generation of neurons from samples of different patients and study the disease or test pharmaceuticals on it. Even though those results are another step to clinical application, there is still a long way to go, says David Vilchez: “New neurons from the dish could be important for studying diseases like Parkinson´s, Alzheimer´s or Huntington´s, but we are still at the starting point of this exciting research.” The research was a collaboration between CECAD, the Center for Molecular Medicine Cologne and the University Clinic Cologne.

Source:

https://www.portal.uni-koeln.de/9015.html?&L=1&tx_news_pi1%5Bnews%5D=4731&tx_news_pi1%5Bcontroller%5D=News&tx_news_pi1%5Baction%5D=detail&cHash=0f48b2a168f0984e8c7b546cbb939fae

879d1a40-6ccd-40fe-a528-248447605dbf|0|.0

Posted in: Cell Biology | Life Sciences News | Medical Condition News

Tags: Cell, CRISPR, Embryonic Stem Cell, Embryonic Stem Cells, Gene, Gene-Editing, Neurodegenerative Diseases, Neurogenesis, Nucleus, Protein, Skin, Somatic Cells, Stem Cell

Tagged with:

About author

Related Articles