Breaking News
May 20, 2018 - Why drug users prefer heroin at home, but cocaine while out
May 20, 2018 - Gene therapy that reverses blindness in dogs could also help treat humans
May 20, 2018 - Opioid-Related Payments Linked to Increase in Opioid Rx
May 20, 2018 - Phone apps push people to take their pills
May 20, 2018 - Backbreaking Work May Shorten Men’s Lives
May 20, 2018 - Harsher drug laws won’t stop violence, argues former police chief
May 20, 2018 - Cognitive decline in dementia is not reduced by exercise
May 20, 2018 - Detecting breast cancer with non-invasive ‘disease screening pill’
May 20, 2018 - Simple treatment may minimize hearing loss triggered by loud noises
May 20, 2018 - Alignment of mother and offspring body clock could prevent diseases such as heart disease and obesity
May 20, 2018 - New commercial data warehouse for life sciences
May 20, 2018 - Practice Intervention Targeting IV Opioids May Cut Exposure
May 20, 2018 - New study provides insight into blood signatures of inflammation
May 20, 2018 - Scientists make breakthrough discovery about vitamin B12
May 20, 2018 - What Causes Cancer? Misconceptions Abound
May 20, 2018 - Proper burial of dead cells limits inflammation
May 20, 2018 - Study offers novel solution to suppress metastatic spread of deadly breast cancer
May 20, 2018 - Perspectives of patients and caregivers on care transitions
May 20, 2018 - Guidelines for weight gain in pregnancy should be changed for underweight and very obese women
May 20, 2018 - Researchers transplant retinal sheets derived from human embryonic stem cells in retinal degeneration mouse models
May 20, 2018 - U.S. military personnel at greater risk for skin cancer than general population
May 20, 2018 - Your immune system holds the line against repeat invaders, thanks to this molecule
May 20, 2018 - Between death and deportation
May 20, 2018 - Developing a High Throughput Mass Spectrometry Platform for Drug Discovery
May 19, 2018 - New project aims to increase awareness among hospital clinicians of non-beneficial treatment at end-of-life
May 19, 2018 - Automated bone scan index offers accurate, speedy prognostic information about prostate cancer
May 19, 2018 - Rutgers Cancer Institute nurses research various topics to enhance patient experience
May 19, 2018 - Computer models provide valuable insight to structure and function of Ebola, Zika viruses
May 19, 2018 - Study exposes key tactic used by deadly fungus
May 19, 2018 - Bacterial signals are crucial to development of pre-leukemic myeloproliferation, study shows
May 19, 2018 - Global experts identify key issues in supporting children with brain injuries transition back to school
May 19, 2018 - Social connections may protect black men who have sex with men from acquiring HIV
May 19, 2018 - Study IDs Factors Linked to Quality of Life With Dementia
May 19, 2018 - Potassium — Consumer
May 19, 2018 - HIV-1 viruses transmitted at birth are resistant to antibodies in mother’s blood
May 19, 2018 - Some water pitchers are much better at removing toxins, shows research
May 19, 2018 - Scientists discover how unusually long strands of RNA help colon cancer cells avoid death
May 19, 2018 - International study finds viable treatment option for people with mild asthma
May 19, 2018 - Mayo discovery could enable development of personalized ovarian, brain cancer treatments
May 19, 2018 - ‘Superbug’ Surfaces at Poultry Farm in China
May 19, 2018 - UCLA-designed program helps former HIV-positive inmates maintain health after release from jail
May 19, 2018 - New blood test could help avoid more than 40% of prostate biopsies, study finds
May 19, 2018 - Macrophages play key role in maintaining stem cell niche of mammary gland
May 19, 2018 - Ferritin Blood Test: MedlinePlus Lab Test Information
May 19, 2018 - Prolonged exposure to air pollution leads to genetic changes in rat brains, study finds
May 19, 2018 - Scientists identify new potential target to combat acute myeloid leukemia
May 19, 2018 - Ovarian cancer therapy may help treat patients with aggressive pancreatic cancer
May 19, 2018 - MediciNova Announces Opening of Investigational New Drug Application for MN-166 (ibudilast) in Glioblastoma
May 19, 2018 - Research shows that sexual activity and emotional closeness are unrelated to the rate of cognitive decline
May 19, 2018 - Nuffield Council on Bioethics outlines ethical issues arising from use of AI in healthcare
May 19, 2018 - Pancreatic cancer patients with BRCA1 or BRCA2 mutations fare better on platinum-based chemotherapy
May 19, 2018 - Tradeshow Talks with nal von minden Ltd
May 19, 2018 - Chemist begins new project to develop carbohydrate mimics to assist cancer therapies
May 19, 2018 - Healthy Lifestyle for Healthy Older Adults
May 19, 2018 - Epstein-Barr virus linked to seven serious diseases
May 19, 2018 - Biochemists isolate protein supercomplex from bacteria that generates voltage
May 19, 2018 - Mindfulness-based Stress Management Course for cancer survivors
May 19, 2018 - Simple screening tool can help identify people at increased risk for dementia
May 19, 2018 - Many gay and bisexual teenage boys use adult hookup apps to connect with friends, partners
May 19, 2018 - Maternal Exposure to Polluted Air Tied to Elevated Child BP
May 19, 2018 - Experimental drug extends survival in progeria
May 19, 2018 - FutureNeuro and GreenLight Medicines team up to develop cannabis-based treatments for epilepsies
May 19, 2018 - Brazilians with less education more likely to perceive as being in poor health
May 19, 2018 - Experts available to discuss subjects related to Ebola virus
May 19, 2018 - Opioid Makers’ Perks to Docs Tied to More Prescriptions
May 19, 2018 - AI detects patterns of gut microbes for cholera risk
May 19, 2018 - Researcher develops small molecules that inhibit derailment of gene expression in cancer
May 19, 2018 - Study brings fresh insights into biology of malaria parasite
May 19, 2018 - Microglia may play key role in slowing progression of prion diseases, research suggests
May 19, 2018 - Safety program successfully reduces SSIs in patients undergoing colorectal operations
May 19, 2018 - Clinical study supports usual medical care plus chiropractic care for low back pain
May 19, 2018 - Vermont legislators pass a drug importation law. So what?
May 19, 2018 - Trump proposes cutting planned parenthood funds. What does that mean?
May 19, 2018 - Cognitive training intervention reduces gait freezing in Parkinson’s patients, study shows
May 19, 2018 - Clinical trial begins enrollment to test safety of experimental MERS treatments
May 19, 2018 - Scientists disrupt two enzymes to shrink cancer cells
May 19, 2018 - 3D simulation reveals beneficial impact of medically induced brain cooling
May 19, 2018 - Natural antioxidant bilirubin may provide cardiovascular benefits
May 19, 2018 - Lash Group introduces latest solution for electronic benefit verification
May 19, 2018 - New CRISPR/Cas9 system targets regulatory genes of AIDS virus
Cardiomyocytes fuse transiently during heart development and regeneration

Cardiomyocytes fuse transiently during heart development and regeneration

image_pdfDownload PDFimage_print

Cardiomyocytes fuse during cardiac development and regeneration. A scientist of the German Centre for Cardiovascular Research (DZHK) discovered these previously unknown processes with the aid of genetically modified zebrafish in which fused cells fluoresce.

Cells fuse for different reasons. It is well known that skeletal muscle fibres develop from fused myoblasts. Cell fusion also seems to play a role in diseases such as cancer. Reprogramming differentiated cells through fusion and thus stimulating their growth is moreover a therapeutic vision for many diseases. Yet the mechanisms that underlie fusion are not well understood and it is unclear how significant this process is. Ultimately, there has previously been a lack of suitable models and tools to observe fusion in living organisms under normal conditions.

Cardiomyocytes fuse transiently

The DZHK scientist Dr. Suphansa Sawamiphak and colleagues at the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC) in Berlin and at the Max Planck Institute for Heart and Lung Research in Bad Nauheim have now succeeded in genetically modifying zebrafish in such a way that they can reliably identify cell fusion in the living organism. They named their system Fluorescence Activation after Transgene Coupling, or FATC for short. In this system, a fluorescent reporter gene is only read when cells fuse together. These cells glow under the fluorescence microscope. Using this, they observed for the first time that cardiomyocytes in fish fuse. This fusion is transient: the cells fluoresce only for as long as they are fused together. When they separate, the luminous signal is lost.

“What is special about our system is that we can render fusion visible throughout the body”, says Sawamiphak. “Previously, cell fusion could only be observed if tagged cells, such as bone marrow cells, were transplanted and whether the cells fuse in specific tissues was then tracked. Tissue and cell type were therefore predetermined; unknown fusion processes thus remained concealed.”

Unexpected process during development

Damaged zebrafish hearts are capable of regenerating fully. The Berlin-based researchers now observed that cardiomyocytes fuse during this phase. Moreover, they could detect the occurrence of this process during the development of the fish larvae. “We expected fusion during the regeneration of the damaged heart. However, that it occurs during development was very surprising”, reports Sawamiphak.

Cell growth is linked to fusion

Further investigations showed that the proportion of fused cells dropped by more than half in the adult fish hearts in comparison to the larval hearts. “The healthy adult myocardium also only has a low cell growth”, says Sawamiphak. “For this reason, we investigated whether transient cell fusion is linked to mitotic activity.” The findings show that the cardiomyocytes which experienced membrane fusion represent a highly proliferative subpopulation in the developing heart. The researchers could also observe this link between cell growth and cell fusion in the regenerating adult fish hearts. Following an injury, the cell proliferation rate generally increases enormously. And in the genetically modified zebrafish, the fluorescent and thus fused cells constituted a significant proportion of these proliferative cells in the heart. Sawamiphak suspects that transient fusion prepares cardiomyocytes for proliferation.

Understanding and using regeneration

Next, the DZHK scientist and her team intend to ascertain how exactly cardiomyocyte fusion and mitotic activity are correlated, and analyse the mechanisms of cell fusion. “Our findings show fundamental mechanisms in zebrafish. We thus hope to better understand the distinct regenerative capacity of the zebrafish heart and provide new avenues for repairing the damaged mammalian heart”, Sawamiphak is looking to the future.

Source:

https://dzhk.de/en/news/latest-news/article/cardiomyocytes-fuse-when-the-heart-grows-and-regenerates/

Tagged with:

About author

Related Articles