Breaking News
February 20, 2018 - Researchers discover that activation of specific enzyme may help suppress tumor metastasis
February 20, 2018 - Blood or marrow transplantation survivors have higher risk of cognitive impairment
February 20, 2018 - Booze Beats Pot at Being Unhealthy: Oregon Poll
February 20, 2018 - Morning Break: ’20 Years Late’; Drugs in the Dirt; Catching Flu in the Dorm
February 20, 2018 - Another piece to the puzzle in naked mole rats’ long, cancer-free life
February 20, 2018 - Scientists identify four viruses that can produce insulin-like hormones
February 20, 2018 - New e-Health solution developed to prevent cardiovascular disease, dementia in senior citizens
February 20, 2018 - New genetic risk score could help guide screening decisions for prostate cancer
February 20, 2018 - Study finds higher risk of stroke among blacks with atrial fibrillation than whites
February 20, 2018 - Physical activity could be used as strategy for diabetes prevention
February 20, 2018 - Researchers develop sensing method for early detection of cancer and diabetes
February 20, 2018 - New wearable electronics could be game-changer for stroke rehabilitation
February 20, 2018 - Immune history influences person’s response to flu vaccine
February 20, 2018 - Serenity Now! Learn to Have Patience with Patients
February 20, 2018 - Computer simulation addresses the problem of blood clotting
February 20, 2018 - Persistent bloating can be a sign of ovarian cancer, warns charity
February 20, 2018 - Key protein involved in epigenetic regulation of gene expression guides skin cell renewal
February 20, 2018 - Heart attack symptoms often missed in women
February 20, 2018 - Diagnosis of celiac disease takes 3.5 years for patients who do not report GI symptoms
February 20, 2018 - Study reveals functional dynamics of ion channels
February 20, 2018 - Study explores link between mortality risk and combustible tobacco use
February 20, 2018 - ‘She Trusted Me, and I’d Turned Her Away’
February 20, 2018 - AbbVie and Voyager Therapeutics collaborate to develop new treatments for tauopathies
February 20, 2018 - Fast food makes the immune system more aggressive in the long term
February 20, 2018 - Therapeutic target for glaucoma could have treatment ramifications for Alzheimer’s and Parkinson’s
February 20, 2018 - Overcoming Negative Reviews | Medpage Today
February 20, 2018 - MyD88—villain of allergies and asthma
February 20, 2018 - Food scientists develop rapid screening technique to detect pesticide residue in vegetables
February 20, 2018 - Lab-grown cerebellar cells may help explain how ASD develops at molecular level
February 20, 2018 - Scientists explore connection between bad sleep habits and stiff blood vessels
February 20, 2018 - New Treatment Apalutamide (Erleada) Approved for Prostate Cancer That Resists Hormone Therapy
February 20, 2018 - Do You Really Need My Signature on That?
February 20, 2018 - HIV-1 genetic diversity is higher in vaginal tract than in blood during early infection
February 20, 2018 - Diabetes does not increase work-loss years due to early retirement
February 20, 2018 - Researchers aim to find out how PTSD affects decisions of police
February 20, 2018 - UH Cleveland Medical Center explores novel treatments for uterine fibroids
February 20, 2018 - Flu Vax Efficacy 25% Against Predominant H3N2 Strain So Far
February 20, 2018 - HIV screening most optimal at 25 years of age if no risk factors
February 20, 2018 - Loyola Medicine primary care physician offers advice to minimize risk of flu
February 20, 2018 - Safe sleep recommendations for parents that may help reduce child’s risk of SUID
February 20, 2018 - Why Do So Few Docs Have Buprenorphine Waivers?
February 20, 2018 - Low levels of alcohol good for the brain
February 20, 2018 - Experimental treatment improves invisible symptoms of a man with spinal cord injury
February 20, 2018 - Myriad’s EndoPredict offers better prediction of breast cancer recurrence, analysis shows
February 20, 2018 - Researchers identify fifteen genes that determine our facial features
February 20, 2018 - Morning Break: New Health IT Player; Luxturna No Bargain; Nuclear Freakout
February 20, 2018 - How does it compare? Hospice care at home, at assisted living facility, at nursing home
February 19, 2018 - Scientists develop water-soluble warped nanographene for bioimaging
February 19, 2018 - It’s Not Your Imagination: You’re Hungrier After Losing Weight
February 19, 2018 - Antihypertensive Use At Delivery Rising in Preeclampsia
February 19, 2018 - A centuries-old math equation used to solve a modern-day genetics challenge
February 19, 2018 - Liquid biopsies could be used as new predictive marker for metastatic TNBC
February 19, 2018 - Russian researchers develop new multi-layered biodegradable scaffolds
February 19, 2018 - Are ‘Vaccine Skeptics’ Responsible for Flu Deaths?
February 19, 2018 - Hidden genetic effects behind immune diseases may be missed, study suggests
February 19, 2018 - Emergency nurses experience regular verbal and physical abuse
February 19, 2018 - Study sheds light on biology that guides behavior across different stages of life
February 19, 2018 - Morning Break: Transgender Breast Feeding; Brazilian ‘Pro-Vaxxers’; Post-Stroke Exercise
February 19, 2018 - Meningitis vaccination strategy in Africa found to be effective, economical
February 19, 2018 - Researchers uncover how excess calcium may influence development of Parkinson’s disease
February 19, 2018 - Psoriasis drug also effective at reducing aortic inflammation
February 19, 2018 - Excess emissions can make serious contributions to air pollution, study shows
February 19, 2018 - Researchers reveal potential biological roots behind individuality
February 19, 2018 - Diabetes Drugs Differ on HF; School-Based Obesity Program Flop; Plaque Type in ACS
February 19, 2018 - Surgical infections linked to drug-resistant bugs, study suggests
February 19, 2018 - Poor awareness may hinder a child’s early dental care
February 19, 2018 - Research finds rising trend in incidence of merkel cell carcinoma
February 19, 2018 - Researchers uncover Ras protein’s role in uncontrolled cancer growth
February 19, 2018 - FDA Approves Apalutamide (Erleada) to Help Curb a Tough-to-Treat Prostate Cancer
February 19, 2018 - Educational Tool Boosts Cervical Length Screening
February 19, 2018 - Spider’s web inspires removable implant that may control type 1 diabetes
February 19, 2018 - Scientists develop fluorescent probe to identify cancer stem cells
February 19, 2018 - University Hospital of Santiago de Compostela participates in large pancreatic cancer study
February 19, 2018 - New blood test shows promise to revolutionize diagnosis of tick-borne diseases
February 19, 2018 - Report: Use, Not Price, Drives State Health Costs
February 19, 2018 - Emergency services crews often unprepared for diabetic crises
February 19, 2018 - Scientists in Sweden create DNA nanowires that offer hope for treatment of diseases
February 19, 2018 - ID Break: Clean Hands, Fewer Abx; $11 Million HIV Cure?; MenB Vax for Kids
February 19, 2018 - Patient exposure to X-rays depends on how dentists are paid
February 19, 2018 - Study reveals parents’ views toward children’s tanning bed use
Cardiomyocytes fuse transiently during heart development and regeneration

Cardiomyocytes fuse transiently during heart development and regeneration

image_pdfDownload PDFimage_print

Cardiomyocytes fuse during cardiac development and regeneration. A scientist of the German Centre for Cardiovascular Research (DZHK) discovered these previously unknown processes with the aid of genetically modified zebrafish in which fused cells fluoresce.

Cells fuse for different reasons. It is well known that skeletal muscle fibres develop from fused myoblasts. Cell fusion also seems to play a role in diseases such as cancer. Reprogramming differentiated cells through fusion and thus stimulating their growth is moreover a therapeutic vision for many diseases. Yet the mechanisms that underlie fusion are not well understood and it is unclear how significant this process is. Ultimately, there has previously been a lack of suitable models and tools to observe fusion in living organisms under normal conditions.

Cardiomyocytes fuse transiently

The DZHK scientist Dr. Suphansa Sawamiphak and colleagues at the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC) in Berlin and at the Max Planck Institute for Heart and Lung Research in Bad Nauheim have now succeeded in genetically modifying zebrafish in such a way that they can reliably identify cell fusion in the living organism. They named their system Fluorescence Activation after Transgene Coupling, or FATC for short. In this system, a fluorescent reporter gene is only read when cells fuse together. These cells glow under the fluorescence microscope. Using this, they observed for the first time that cardiomyocytes in fish fuse. This fusion is transient: the cells fluoresce only for as long as they are fused together. When they separate, the luminous signal is lost.

“What is special about our system is that we can render fusion visible throughout the body”, says Sawamiphak. “Previously, cell fusion could only be observed if tagged cells, such as bone marrow cells, were transplanted and whether the cells fuse in specific tissues was then tracked. Tissue and cell type were therefore predetermined; unknown fusion processes thus remained concealed.”

Unexpected process during development

Damaged zebrafish hearts are capable of regenerating fully. The Berlin-based researchers now observed that cardiomyocytes fuse during this phase. Moreover, they could detect the occurrence of this process during the development of the fish larvae. “We expected fusion during the regeneration of the damaged heart. However, that it occurs during development was very surprising”, reports Sawamiphak.

Cell growth is linked to fusion

Further investigations showed that the proportion of fused cells dropped by more than half in the adult fish hearts in comparison to the larval hearts. “The healthy adult myocardium also only has a low cell growth”, says Sawamiphak. “For this reason, we investigated whether transient cell fusion is linked to mitotic activity.” The findings show that the cardiomyocytes which experienced membrane fusion represent a highly proliferative subpopulation in the developing heart. The researchers could also observe this link between cell growth and cell fusion in the regenerating adult fish hearts. Following an injury, the cell proliferation rate generally increases enormously. And in the genetically modified zebrafish, the fluorescent and thus fused cells constituted a significant proportion of these proliferative cells in the heart. Sawamiphak suspects that transient fusion prepares cardiomyocytes for proliferation.

Understanding and using regeneration

Next, the DZHK scientist and her team intend to ascertain how exactly cardiomyocyte fusion and mitotic activity are correlated, and analyse the mechanisms of cell fusion. “Our findings show fundamental mechanisms in zebrafish. We thus hope to better understand the distinct regenerative capacity of the zebrafish heart and provide new avenues for repairing the damaged mammalian heart”, Sawamiphak is looking to the future.

Source:

https://dzhk.de/en/news/latest-news/article/cardiomyocytes-fuse-when-the-heart-grows-and-regenerates/

Tagged with:

About author

Related Articles