Breaking News
August 16, 2018 - Ablating the mutant p53 gene in mice with colorectal cancer inhibits tumor growth
August 16, 2018 - Higher BMI in people with prediabetes related to evening preference and lack of sufficient sleep
August 16, 2018 - Using peripheral nerve blocks to treat facial pain may produce long-term pain relief
August 16, 2018 - Neural stem cells are the key to tail regeneration
August 16, 2018 - Study compares genetic and neural contributions to ADHD in children with or without TBI
August 16, 2018 - Adding energy drinks to alcohol may exacerbate negative effects of binge drinking
August 16, 2018 - Eye Examination Can Help Detect Abuse in Children
August 16, 2018 - Know the Difference: Rheumatoid Arthritis or Osteoarthritis?
August 16, 2018 - From ‘sea of mutations,’ two possible cancer links rise to the surface
August 16, 2018 - Does medical school take too long?
August 16, 2018 - Brown University researchers reveal key physical properties of ‘giant’ cancer cells
August 16, 2018 - Regular resistance training improves exercise motivation
August 16, 2018 - Feds urge states to encourage cheaper plans off the exchanges
August 16, 2018 - Seven activities that prevent you from getting quality sleep during summer
August 16, 2018 - Five ways to tell if your baby is getting enough milk from breastfeeding
August 16, 2018 - From Pigs to Peacocks, What’s Up With Those ‘Emotional-Support Animals’?
August 16, 2018 - Breast cancers enlist the help of normal cells to help them spread and survive
August 16, 2018 - Engaging with “high-need” patients outside the clinic
August 16, 2018 - Research illuminates how online forum may offer suicide prevention support for males
August 16, 2018 - Researchers identify way to grow immune cells at large scale for preventing cancer reoccurrence
August 15, 2018 - Keck Medicine of USC’s hospitals ranked among nation’s best for the 10th consecutive year
August 15, 2018 - Researchers compare existing approaches for automating diagnostic procedures of skin lesions
August 15, 2018 - Autism risk determined by health of mom’s gut, research reveals
August 15, 2018 - WELL for Life challenges you to explore the great outdoors
August 15, 2018 - ‘Zombie’ gene protects elephants from cancer, study finds
August 15, 2018 - Ebola outbreak in Congo spreads to active combat zone
August 15, 2018 - Study highlights pollution exposure of babies in prams
August 15, 2018 - Study provides insight into link between sleep apnea and lipid metabolism
August 15, 2018 - New study focuses on promise of gene therapy for Amish nemaline myopathy
August 15, 2018 - Researchers discover new approach to alleviate chronic itch
August 15, 2018 - Uncovering the Mysteries of MS: Medical Imaging Helps NIH Researchers Understand the Tricky Disease
August 15, 2018 - Autistic people at greater risk of becoming homeless – new research
August 15, 2018 - New imaging technique can spot tuberculosis infection in an hour
August 15, 2018 - Scientists study effects of eating breakfast versus fasting overnight before exercise
August 15, 2018 - Talking with children about suicide could save lives
August 15, 2018 - Grip strength of children predicts future cardiometabolic health
August 15, 2018 - Innovative oncofertility program launched by RMA of New York and Mount Sinai Health System
August 15, 2018 - Study shows efficacy, safety of AAV5-based gene therapy to treat sheep model of achromatopsia
August 15, 2018 - Simple score helps predict which hospitalized heart attack patients are at high risk of readmissions
August 15, 2018 - New discoveries show how protein droplets do more than keep cells’ interiors tidy
August 15, 2018 - Study shows impact of optimizing airport flight patterns on human health
August 15, 2018 - Life experiences of feeling unwanted or unplanned associated with attachment insecurity
August 15, 2018 - ACS Briefing Discusses Use of Lessons From Combat Care
August 15, 2018 - Study identifies distinct origin of ADHD in children with history of brain injury
August 15, 2018 - IgG3 antibody stops B cells from fighting pathogens in HIV patients
August 15, 2018 - Scientists discover key vulnerability of mixed lineage leukemia
August 15, 2018 - College students may experience pressures from secondary exposure to opioid abuse
August 15, 2018 - Powerful new microscope reveals inner workings of human cells with unprecedented clarity
August 15, 2018 - Married people who fight nastily more likely to suffer from leaky guts, study suggests
August 15, 2018 - Working Out After Baby – Drugs.com MedNews
August 15, 2018 - Rheumatoid Factor (RF) Test: MedlinePlus Lab Test Information
August 15, 2018 - ADHD linked to an increased risk of injury in children, study finds
August 15, 2018 - UIC researchers receive NIH funding to develop a better way to regenerate bone or tissues
August 15, 2018 - Study reveals how immune cells in the brain influence sexual behavior
August 15, 2018 - Researchers move closer to finding potential soft spot in drug-resistant tuberculosis
August 15, 2018 - Real-time dynamic monitoring of cell’s nucleus for effective cancer screening
August 15, 2018 - Lower rates of Medicare preventive care visits found in racial, ethnic minority older adults
August 15, 2018 - Scientists identify stress hormone as key factor in failure of immune system to inhibit leukemia
August 15, 2018 - Cytoplan introduces three new nutritional supplements
August 15, 2018 - Effective hemorrhage control critical for survival after motorsport accidents
August 15, 2018 - Sygnature Discovery announces ambitious expansion plan with addition of Alderley Park facility
August 15, 2018 - Dietary carbohydrates could lead to osteoarthritis, new study finds
August 15, 2018 - Male tobacco smokers have decreased number of cannabinoid CB1 receptors, study reveals
August 15, 2018 - Scientists explore ways for drug therapies to reach deadly brain tumors
August 15, 2018 - Rethinking fundamental rule of stroke care: ‘Time is brain!’
August 15, 2018 - Scientists reveal role of ‘junk DNA’ in cancer dissemination
August 15, 2018 - Google’s DeepMind AI could soon be diagnosing eye conditions
August 15, 2018 - Scientists trick the brain to embody the prosthetic limb
August 15, 2018 - Researchers focus on uncoupling obesity from diabetes
August 15, 2018 - Clinical study shows how EarlySense system effectively detects opioid-induced respiratory depression
August 15, 2018 - A class of proteins shown to be effective in reducing drug-seeking behaviors
August 15, 2018 - FundamentalVR launches first-of-its-kind SaaS software platform for surgical simulation
August 15, 2018 - Gemphire Announces Termination of Phase 2a Clinical Trial of Gemcabene in Pediatric NAFLD
August 15, 2018 - Rheumatoid arthritis in pregnancy associated with low birth weight and premature birth
August 15, 2018 - Study may help increase effectiveness of antibiotics against drug-resistant bacteria
August 15, 2018 - Analyzing resident-to-resident incidents in dementia may hold the key to reducing future fatalities
August 15, 2018 - Robotic walking frame aims to help maintain mobility of older adults
August 15, 2018 - Simple intervention during routine care reduces alcohol consumption in men with HIV
August 15, 2018 - Genetics Home Reference: gout
August 15, 2018 - Scientists ID genesis of disease, focus efforts on shape-shifting tau
New technique aims to expand understanding of mechanical forces existing between cells

New technique aims to expand understanding of mechanical forces existing between cells

image_pdfDownload PDFimage_print

A new technique that uses tiny elastic balls filled with fluorescent nanoparticles aims to expand the understanding of the mechanical forces that exist between cells, researchers report. A University of Illinois-led team has demonstrated the quantification of 3-D forces within cells living in petri dishes as well as live specimens. This research may unlock some of the mysteries related to embryonic development and cancer stem cells, i.e., tumor-repopulating cells.

For decades, scientists have struggled to quantify the forces, called tractions, that push, pull and squeeze cells throughout their lifecycles. The tools available to measure force were not small enough to fit into intercellular spaces or sensitive enough to detect the miniscule movements within cell colonies.

Although small on a human scale, the magnitudes of these mechanical forces are far from trivial at the cellular level. According to the new study, prior research by the Illinois group and others indicates that traction plays a fundamental role in cell physiology.

The team led by mechanical science and engineering professor Ning Wang reported their findings in the journal Nature Communications.

“If we place a single cell in a medium within a petri dish it will not survive for long, even if we provide all of the nutrients needed,” Wang said. “The cells fail to form any sort of tissue because there is no support or scaffolding on which to build.”

As cells grow and reproduce, they exert forces on each other while competing for space. The team found that if they inject their tiny elastic spheres into early stage embryos of zebrafish and colonies of melanoma cells of mice in petri dishes, they too experience the forces.

“The cells do not seem to mind the intrusion,” Wang said. “The spheres are made of a nontoxic microgel and even though the cells will push them around, they do not seem to interfere with development.”

To measure the amount of force imposed on the cells, the team placed fluorescent nanoparticles inside of the spheres. When the cells squeeze the spheres, the nanoparticles all move the same amount per area of force. The researchers can then measure the motions of the glowing particles using fluorescent light microscopy to calculate the amount of force exerted on the spheres and cells. Using this technique, the team has marked the first successful measurement of all three types of force – compression, tension and shear – in all three dimensions, Wang said.

This ability to quantify force in cells may be very important to cancer cell research, Wang said. The team found that when melanoma tumor cells of mice in vitro begin to reproduce from a single cell to about 100 to 200 cells, compressive stress does not increase.

“We thought that cancer cells would generate more pressure at this early growth stage while the mass of the tumor increases, as we observed in zebrafish embryos, but they do not,” Wang said. “We suspect that the cancer cells begin to spread out or metastasize right after this stage.”

Primary tumors are usually not deadly, Wang said. The real killer appears to be the spread of tumor-repopulating cells from primary tumors into soft tissues – with low intercellular tractions – like bone marrow, brain, lung and liver. “Although the underlying mechanism for metastasis is unclear, we have hypothesized that tumor-repopulating cells spread very rapidly in these secondary soft tissues. Having the ability to measure changes in tractions at the intercellular level may serve as an early cancer-detection tool,” Wang said.

This microgel sphere technology may also help unravel the mechanisms behind a metastasis-halting synthetic drug recently described by Wang and his colleagues. In addition, Wang’s co-authors are continuing to apply this technology to stem and embryonic cell research. “When other researchers see this powerful new tool that we have developed, they will be excited to use it in many different cell physiology, development and disease applications,” Wang said.

Tagged with:

About author

Related Articles