Breaking News
March 20, 2019 - FDA Approves Zulresso (brexanolone) for the Treatment of Post-Partum Depression
March 19, 2019 - How it manipulates us to tribalism
March 19, 2019 - How can doctors encourage patients to adopt healthier behaviors?
March 19, 2019 - Meet Hal: He's One Sick Robot
March 19, 2019 - Blood test and mathematical model can estimate preterm birth rate in low-resource countries
March 19, 2019 - TAVR procedure safe in patients with unusual valve anatomy
March 19, 2019 - Proteins in the eye may be potential source for cost-effective test to predict Alzheimer’s disease
March 19, 2019 - Opioid Prescriptions Dropped for New Users From 2012 to 2017
March 19, 2019 - New method may better predict the best treatment for burn wounds
March 19, 2019 - “Asian” isn’t specific enough for health data, research suggests
March 19, 2019 - ColumbiaDoctors Presents Honors for Outstanding Commitment to Patient Safety
March 19, 2019 - Innovative model identifies primate species with potential to transmit Zika in the Americas
March 19, 2019 - One-off surgery could offer hope to patients with high blood pressure
March 19, 2019 - How to Protect Your Kids From Drowning
March 19, 2019 - CEA Test: MedlinePlus Lab Test Information
March 19, 2019 - Two years after face transplant, Andy Sandness’ smile shows his progress
March 19, 2019 - Registration now open for Stanford’s Big Data in Precision Health conference
March 19, 2019 - Gene Keeps Fear at Bay, But Only in Females
March 19, 2019 - GARDP and Evotec partner to tackle growing threat of antimicrobial resistance
March 19, 2019 - Ultrasound offers precise, minimally invasive way to measure cardiac output in children
March 19, 2019 - Study suggests potential new approach to treat atopic dermatitis
March 19, 2019 - Sense of control over life makes older adults feel younger
March 19, 2019 - Study shows how probiotics influence gut microbiota
March 19, 2019 - Study offers new evidence that narcolepsy is an autoimmune condition
March 19, 2019 - Breastfeeding may offer long-term heart health benefits for women
March 19, 2019 - Study of young athletes suggests snoring and sleep apnea are linked to sudden cardiac death
March 19, 2019 - Did Your Doctor ‘Ghost’ You? An Employment Contract May Be To Blame
March 19, 2019 - Food pantry clients more likely to make healthy choices when meal kits and recipe tastings are available
March 19, 2019 - Mental health problems among children increasing
March 19, 2019 - New ISO standard helps evaluate and manage impact of environmental damage
March 19, 2019 - CardioMEMS heart failure sensor reliably safe, effectively reduces hospitalizations
March 19, 2019 - Researchers report promising results of potential reversal agent
March 19, 2019 - Scientists identify brain circuit responsible for cocaine-seeking behavior during relapse
March 19, 2019 - First African-American Neuroscience Research Initiative launched to close the gap in health disparities
March 19, 2019 - Bimekizumab Demonstrated Long-Term Maintenance of Complete or Almost Complete Skin Disease Resolution for Psoriasis Patients in BE ABLE 2 Extension Study
March 19, 2019 - Attitudes about health affect how older adults engage with negative health news
March 19, 2019 - Huron Digital Pathology to unveil new ‘Scan Index Search’ platform at USCAP 2019
March 19, 2019 - Frequent intake of sugary drinks tied to greater risk of premature death
March 19, 2019 - Bruker showcases new analytical systems and applied market solutions at Pittcon 2019
March 19, 2019 - Framingham cardiovascular risk prediction model from the 1990s still gives the best results
March 19, 2019 - New article focuses on integrative health, value-based medicine, and whole systems research
March 19, 2019 - Foamix Announces FDA Acceptance of New Drug Application for FMX101 Minocycline Foam for the Treatment of Moderate-to-Severe Acne
March 19, 2019 - National survey of emergency dept management of self-harm highlights successes, room for improvement
March 19, 2019 - Scientists reverse alcohol-seeking behavior in rats with flip of a switch
March 19, 2019 - Researchers hope blood test that accurately diagnoses fibromyalgia could be available within five years
March 19, 2019 - New Planmeca ProScanner 2.0 offers fast and dependable intraoral imaging
March 19, 2019 - A new option for reducing LDL cholesterol in patients at high risk for heart attack, stroke
March 19, 2019 - Common medications to treat heartburn linked to increased risks for kidney failure
March 19, 2019 - Current HBV genome sequences help deduce ancient human population movements into Australia
March 19, 2019 - Pure omega-3 prescription drug significantly reduces the occurrence of ischemic events
March 19, 2019 - Researchers use big data to gain better understanding of hepatitis E virus
March 19, 2019 - Use of synthetic psychedelic linked to improvements in depression and anxiety
March 19, 2019 - GARP protein can be a potential target for immunotherapy against colorectal cancer
March 19, 2019 - Knee Pain Not Tied to Activity Levels in Knee Osteoarthritis
March 19, 2019 - Study shows benefits of delayed cord clamping in healthy babies
March 19, 2019 - Pharmacists can undertake overall clinical responsibility for patients, shows study
March 19, 2019 - A cell’s “self-destruct” function could yield new therapies
March 19, 2019 - Latest advances and perspectives of all AI types used in pharmaceutical R&D
March 19, 2019 - Prophylactic cranial irradiation used as standard approach for patients with NSCLC
March 19, 2019 - Sugar-sweetened beverages may be linked with increased risk of cardiovascular mortality
March 19, 2019 - AHA News: Black Woman in Their 50s Face Especially High Stroke Risk
March 19, 2019 - Secrets of early life revealed from less than half a teaspoon of blood
March 19, 2019 - Immune cells engineered to tattle on suspicious cells in the body
March 19, 2019 - Heart attack patients who are taken to heart care centres directly survive longer
March 19, 2019 - IVF babies have increased in birthweight over the past 25 years, study reveals
March 19, 2019 - Study highlights the need for psychiatric care to be integrated into cancer treatment
March 19, 2019 - Testosterone treatment lowers recurrence rates in low-risk prostate cancer patients
March 19, 2019 - Caterpillars could hold the secret to new treatment for Osteoarthritis
March 19, 2019 - Parkinson’s treatment delivers a power-up to brain cell ‘batteries’
March 19, 2019 - Stanford launches new Institute for Human-Centered Artificial Intelligence
March 19, 2019 - Wireless earphones may cause cancer
March 18, 2019 - ACC/AHA guideline for prevention of cardiovascular disease released
March 18, 2019 - UTA nursing professor receives $6.575 million to attack musculoskeletal diseases
March 18, 2019 - Gene medication shows promise to treat spinal cord injuries
March 18, 2019 - First Human Study of “Robotic” RaniPill™ Capsule to Replace Injections Announced by Rani Therapeutics
March 18, 2019 - Food Allergy Testing: MedlinePlus Lab Test Information
March 18, 2019 - Altered brain activity patterns of Parkinson’s captured in mice
March 18, 2019 - Apple Heart Study demonstrates ability of wearable technology to detect atrial fibrillation | News Center
March 18, 2019 - Cardiovascular benefits of diabetes drug extend across a wide spectrum of patients, shows study
March 18, 2019 - Novel cardiac pump shows superior outcomes in patients with advanced heart failure
New chemo-optogenetic method enables multi-directional activity control of cellular processes

New chemo-optogenetic method enables multi-directional activity control of cellular processes

image_pdfDownload PDFimage_print

Cells need for reactions on environmental changes and a balanced system of signaling cascades within the cell. Proteins outside of the cell, on the cellular surface, inside the cellular membrane, and within the cell orchestrate many fine-tuned signaling pathways, which result in adequate reactions on the environmental conditions or changes in the organism itself. The spatio-temporal organization of cellular processes, for example, cell signaling, cell polarization and neurite outgrowth, is often regulated by the subcellular distribution of molecules or organelles.

Individual proteins can perform distinct functions when located at different subcellular locations. One example is Rac1 protein, which controls the shape of the skeleton of the cell at the intracellular plasma membrane, but when it localizes in the nucleus it regulates nuclear morphology. The nucleocytoplasmic shuttling of Rac1 plays an important role in tumor invasion. In neurons, the bidirectional transport along axonal microtubules plays a critical role in the proper subcellular distribution of organelles. Its misregulation is involved in neurodegenerative diseases. However, the analysis of complex processes that involve cycling, trafficking or shuttling of signal molecules/organelles between different cell compartments remains a major challenge.

The group of Yaowen Wu, who became recently professor at the department of Chemistry at Umeå University, has now developed a new technology termed “Multi-directional Activity Control (MAC)”, which makes live studies of cell signaling processes possible. The researchers are pioneers in developing methods for realtime observation of cellular mechanisms under controlled conditions. They used a photoactivatable, dual-chemically induced dimerization (pdCID) system to control the positioning of organelles and proteins at multiple locations in a single cell.

This system combines two chemical reactions that form protein dimers in a single cell. One of them could be controlled by light.

– We showed that our photoactivatable and chemically induced dimerization system could be used to control the function of cellular organelles and cellular signaling pathways in a single cell on a fine-tuned and multilayer level, which was not possible with existing methods before. We combined two modular systems in a parallel or competitive manner to enable multi-directional control over protein or organelle activity by small molecules and light, says Yaowen Wu, who is just setting up his new lab in northern Sweden.

The research group could also show that their new technology enables very rapid induction and observations of different cellular reactions and enable now perturbation studies which have been not possible using traditional genetic approaches.

Using this method, the scientists operated multiple cycles of Rac1 shuttling among the cytosol, plasma membrane and nucleus in a single cell. They could control the transport of peroxisomes (a cellular organelle involved in oxidization of molecules) in two directions, i.e. to the cell periphery and then to the cell body, and vice versa. This is like playing snooker in the cell, but at micrometer scale.

– The MAC approach could also be used to emulate or interfere with disease conditions involving protein/organelle positioning in order to study pathogenic mechanisms, and ultimately aid the development of their therapeutic intervention, the group said in their very important publication in prestigious journal Angewandte Chemie.

Source:

http://www.ucmr.umu.se/news-78310/1653-snooker.html

Tagged with:

About author

Related Articles