Breaking News
November 16, 2018 - AHA: PTSD Common Among Those Who Suffer Tear in the Aorta’s Wall
November 16, 2018 - Many RA patients’ pain related to central nervous system
November 16, 2018 - Changes in Himalayan gut microbiomes linked to diet
November 16, 2018 - Inhibition of prostaglandin E2 enhances ability to combat infectious colitis
November 16, 2018 - Chronic dry eye can slow reading rate and disrupt day to day tasks
November 16, 2018 - Researchers develop new drug molecule that inhibits inflammation
November 16, 2018 - Dementia symptoms peak in winter and spring, study finds
November 16, 2018 - Stanford tobacco researcher weighs in on JUUL
November 16, 2018 - Increasing omega-3 fatty acid intake during pregnancy reduces risk of premature birth, review finds
November 16, 2018 - Researchers find no link between infants waking up at night and later developmental problems
November 16, 2018 - Both parents and children agree about confidential medical services
November 16, 2018 - FDA warns against use of unapproved pain medications with implanted pumps
November 16, 2018 - Precision medicine-based approach to slow or reverse biologic drivers of Alzheimer’s disease
November 16, 2018 - Study provides new insight into norovirus outbreaks, may help guide efforts to develop vaccines
November 16, 2018 - Inexpensive, portable air purifier could help protect the heart from pollution
November 16, 2018 - New 15-minute scan could help diagnose brain damage in babies up to two years old
November 16, 2018 - Deep brain stimulation not effective for treating early Alzheimer’s
November 16, 2018 - Traditional chemotherapy superior to new alternative for oropharyngeal cancers | News Center
November 16, 2018 - What This Pond Protist Does With Its Genome Will Astound You
November 15, 2018 - Researchers develop tool that speeds up analysis and publication of biomedical data
November 15, 2018 - Scientists identify mechanism used by lung cancer cells to obtain glucose
November 15, 2018 - Abnormalities in development of the brain could be involved in onset of autism, finds new study
November 15, 2018 - Soy protein equally effective as animal protein in building muscle strength
November 15, 2018 - American Academy of Pediatrics, Nov. 2-6
November 15, 2018 - Dopamine drives early addiction to heroin
November 15, 2018 - Variance in gut microbiome in Himalayan populations linked to dietary lifestyle | News Center
November 15, 2018 - Reducing Cardiovascular Disease: The Amish Way
November 15, 2018 - King’s researchers launch charter to guide organizations to engage abuse survivors in research
November 15, 2018 - Enable Injections enters into development agreements with UCB and Apellis Pharmaceuticals
November 15, 2018 - TGen North collaborates with NARBHA Institute to advance human health
November 15, 2018 - Researchers discover molecular basis for therapeutic actions of an African folk medicine
November 15, 2018 - Human Cell Atlas study of early pregnancy shows how mother’s immune system is modified
November 15, 2018 - New guidelines for detecting and managing sarcopenia to be launched in the UK
November 15, 2018 - Researchers explore role of dietary composition on energy expenditure
November 15, 2018 - Elsevier launches Entellect™ Platform, unlocking value by creating AI-ready life sciences data
November 15, 2018 - Now that cannabis is legal in Canada, let’s use it to tackle the opioid crisis
November 15, 2018 - In the Spotlight: At the intersection of tech, health, and ethics
November 15, 2018 - Traditional Glaucoma Test Can Miss Severity of the Disease
November 15, 2018 - Researchers directly connect activities of genes with instinctive behavior in male cichlids
November 15, 2018 - Salk researchers report new methods to identify AD drug candidates with anti-aging properties
November 15, 2018 - St. Jude Hospital announces availability of largest collections of leukemia samples
November 15, 2018 - Attenua Announces First Patient Treated in Phase 2 Clinical Trial in Chronic Cough with Bradanicline
November 15, 2018 - Designing a novel cell-permeable peptide chimera to promote wound healing
November 15, 2018 - NEI investigators combine two imaging modalities to view the retina in unprecedented detail
November 15, 2018 - Determining how hearts develop to better understand congenital heart defects
November 15, 2018 - Maverick immune cells can act independently to identify and kill cancer cells, finds research
November 15, 2018 - Advanced AI and big data methods to tackle dementia
November 15, 2018 - Report reveals increase in pancreatic cancer death rates across Europe
November 15, 2018 - Luxia Scientific announces availability of its gut microbiome test in Luxembourg
November 15, 2018 - New diabetes drugs linked to increased risk of lower-limb amputation and ketoacidosis
November 15, 2018 - New approach targets matrix surrounding neurons to protect neurons after stroke
November 15, 2018 - Lilly Submits New Drug Application to the FDA for Lasmiditan for Acute Treatment of Migraine
November 15, 2018 - Heart failure patients shouldn’t stop meds even if condition improves: study
November 15, 2018 - Parents and carers of people with diabetes experience emotional or mental health problems
November 15, 2018 - RetiPharma secures funding to develop new peptide drug for treating degenerative eye disorders
November 15, 2018 - Breakthrough research could lead to a new wave of cancer-fighting antibodies
November 15, 2018 - Mylan and Biocon launch new insulin glargine biosimilar in the UK
November 15, 2018 - For wildfire safety, only particular masks guard against toxic particulate matter
November 15, 2018 - New study of tribe shows influence of Western diet and lifestyle on blood pressure
November 15, 2018 - Scientists harness power of natural killer cells to treat children with neuroblastoma
November 15, 2018 - Investigating foodborne disease outbreak in Bosnia and Herzegovina based on simulation game
November 15, 2018 - Recommendations Issued for Management of Bradycardia
November 15, 2018 - Benefit unclear due to a lack of suitable studies
November 15, 2018 - TAMEST recognizes UT Southwestern’s Ralph DeBerardinis for changing our understanding of cancer
November 15, 2018 - Researchers discover key factors behind intestinal inflammation in CVID patients
November 15, 2018 - CityU develops first microarrayed 3D neuronal culture platform
November 15, 2018 - Expert suggests ways to control uncomfortable vaginal symptoms in diabetic women
November 15, 2018 - New edition of Red Journal focuses on roles of imaging in radiation oncology
November 15, 2018 - Doctors Aren’t Promoting Breastfeeding’s Cancer-Protection Benefit
November 15, 2018 - Collection of demonstration projects highlights value of patient engagement in research
November 15, 2018 - Technique to ‘listen’ to a patient’s brain during tumour surgery
November 15, 2018 - Seven-year-old returns to life as a “normal, healthy child” following bone marrow transplant
November 15, 2018 - AMSBIO expands range of high quality FFPE cancer cell line controls
November 15, 2018 - Marijuana use by kidney donors has no effect on transplant outcomes
November 15, 2018 - Exploring NMR Spectroscopy Applications through Interesting Infographics
November 15, 2018 - Chapman University wins additional $2.9 million NIH grant to study Alzheimer’s disease
November 15, 2018 - Microgel powder reduces infection and promotes healing
November 15, 2018 - Suicidal patients with prescribed access to psychotropic drugs should be closely monitored
November 15, 2018 - Nitric oxide-releasing technology shows potential to reduce healing time of diabetic foot ulcers
November 15, 2018 - Mass shootings may trigger unnecessary blood donations
Researchers examine immune responses to CRISPR-Cas9 gene editing system

Researchers examine immune responses to CRISPR-Cas9 gene editing system

image_pdfDownload PDFimage_print

The CRISPR-Cas9 gene editing system has been generating excitement within the field of gene therapy, inspiring hopes of molecular tools capable of treating genetic diseases. By studying immune responses to CRISPR-Cas9 in humans, researchers from Charité – Universitätsmedizin Berlin have found widespread immunity to the Cas9 protein. The researchers are in the process of developing innovative solutions that will ensure CRISPR-Cas9 can be used safely in a range of clinical applications. Their report on the potential benefits and risks of CRISPR-Cas9 can be found in the current issue of Nature Medicine.

CRISPR-Cas9 is a new molecular gene editing technique that enables scientists to make specific changes to the DNA of humans, other animals and plants. The technique is analogous to a pair of molecular scissors, which enable scientists to cut, silence or replace specific DNA sequences. The CRISPR-Cas9 gene editing technology can be used in vitro or in vivo (i.e. it can be used to modify cells outside the body, or it can be delivered into the body to directly modify specific target cells). Both applications have demonstrated effectiveness and ease of use in animal models.

However, there are insufficient data to allow us to evaluate the risks and benefits of using this technology in humans. By studying immune responses to the CRISPR-Cas9 system, a research team, led by Dr. Michael Schmück-Henneresse of Charité’s Berlin-Brandenburg Center for Regenerative Therapies (BCRT), has succeeded in closing this knowledge gap.

“The Cas9 protein, which is derived from Streptococcus bacteria, forms an integral part of the CRISPR-Cas9 system. As streptococcal infections are common in humans, we hypothesized that there might be a pre-existing immunological memory to Cas9,” explains Dr. Schmück-Henneresse. T cells (human immune cells) that react to Cas proteins were found in almost all of the healthy human subjects tested. Cas molecules derived from other bacteria, such as staphylococci or gastrointestinal bacteria, also generate this type of immune reaction – a phenomenon that may be due to a high degree of similarity between these enzymes. “These types of immune cells can produce unwelcome immune responses during gene therapy, thus potentially impairing the technology’s safety and efficacy. CRISPR-Cas9 may also harbor this risk, and it is something we need to be prepared for,” says the study’s first author, Dimitrios Laurin Wagner, a doctoral student at the BCRT.

This means that cells which have been modified in vitro should not be injected into patients in whom CRISPR-Cas9 remains active. “We have developed a test that will allow us to ensure that cell products can be used with confidence. It can be used to reliably determine whether the risk of an immune reaction is low,” explains Dr. Schmück-Henneresse. However, some genetic diseases produce tissue defects that cannot be modified outside the human body. New solutions must be found to prevent dangerous immune responses to the CRISPR-Cas9 gene editing scissors.

Working with colleagues from the Berlin Center for Advanced Therapies (BeCAT), the researchers have developed a technology that will ensure the safety of CRISPR-Cas9-based therapies. This technology works by isolating and expanding regulatory T cells, which control unwanted immune responses. Initial clinical testing of this technology, used in patients who had undergone either kidney or bone marrow transplants, has produced positive results.

Source:

https://www.charite.de/en/service/press_reports/artikel/detail/sind_wir_immun_gegen_die_genschere_crispr_cas9/

Tagged with:

About author

Related Articles