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Game-Changers in Western Blotting and Protein Analysis

Game-Changers in Western Blotting and Protein Analysis

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An interview with Ramya Prabakaran & Melody Chang from GenScript, discussing the major challenges for researchers carrying out western blotting, and how GenScript’s automated systems are making the technique less time-consuming and more reliable.

How are GenScript involved in neurobiological research?

GenScript offers a wide range of catalog reagents, including cytokines, antibodies as well as peptides, for different areas of neuroscience research. Our MonoRab™ technology generates high-quality rabbit monoclonal antibodies which yield excellent results for Immunohistochemistry (IHC). We design and develop devices with the aim of making research easy.

Antibody - typically used in Western blotting - Tatiana ShepelevaTatiana Shepeleva | Shutterstock

What are the biggest challenges for researchers when conducting a western blot?

Although western blotting is the gold standard, it can be a very time-consuming technique. A typical western workflow can take up to a day or two to complete and get results.

There are also lots of steps involved in western blotting that allow for human error. Unfortunately, due to the nature of the technique, it is often impossible to know that there has been an error until you have imaged the blot.

How are GenScript working to reduce the amount of time it takes to conduct a western blot?

GenScript strives to streamline laboratory protocols so that researchers can attain reliable and reproducible results without compromising time.

For example, eStain L1 is an automated protein staining system developed by GenScript that stains and de-stains 2 mini gels in less than 10 minutes. It also boasts protein staining results that are equivalent or better to traditional staining methods.

Traditional coomassie staining can be time-consuming and messy but eStain L1 offers superior sensitivity compared to conventional methods and can detect as low as 12.5 ng of protein.

We recently launched our eBlot L1 ; the first fast, wet transfer system on the market. Researchers can achieve complete transfer efficiency in 9-17 minutes. Using this system, one of our customers was able to transfer 1200kD in 20 minutes!

Prior to the eBlot, they were performing overnight transfers using the traditional wet tank blotting system. Not only did our system save his lab valuable time, he was also able to obtain superior results in under 20 minutes.

As a world leader in protein analysis, what’s next for the company?

Over 90% of researchers still perform their westerns manually. Even though there are a few automated systems on the market, our research found that they are quite expensive, produce inconsistent results, and didn’t necessarily save researchers time.

We have taken all of this feedback into account and are working on a new automated system that alleviates these issues.

Where can readers find more information?

About GenScript

GenScript is proud to serve the research community since 2002. For the last 16 years, we’ve had the honor of being a reliable partner for life science research and drug discovery industry. Fifteen years of innovation and expertise has allowed GenScript to develop a comprehensive catalog of services and products.

As the world’s leading CRO, GenScript offers a complete list of life science research products and services. Our products include: a broad range of validated catalog antibodies, protein electrophoresis gels and reagents, CRISPR/molecular biology products and more.

Our services include: gene synthesis, custom monoclonal and polyclonal antibody generation, custom protein expression and purification, custom peptide synthesis, and custom cell line development. In addition, GenScript provides preclinical drug development services including in vitro pharmacology, in vivo pharmacology, and antibody/protein engineering services.

At GenScript, we are focused on improving human and animal health by enabling scientists to expedite life science and biomedical research. Powered by innovation, GenScript aims to make scientific research accessible by providing the essential tools necessary to tackle complex problems. We are constantly developing innovative products that incorporate the lastest technologies to address the challenges that researchers face today.

For more information, please visit www.genscript.com or call us at 732-885-9188.

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