Breaking News
May 3, 2019 - Vaping and Smoking May Signal Greater Motivation to Quit
May 3, 2019 - Dementia looks different in brains of Hispanics
May 3, 2019 - Short-Staffed Nursing Homes See Drop In Medicare Ratings
May 3, 2019 - Study of teens with eating disorders explores how substance users differ from non-substance users
May 3, 2019 - Scientists develop new video game that may help in the study of Alzheimer’s
May 3, 2019 - Arc Bio introduces Galileo Pathogen Solution product line at ASM Clinical Virology Symposium
May 3, 2019 - Cornell University study uncovers relationship between starch digestion gene and gut bacteria
May 3, 2019 - How to Safely Use Glucose Meters and Test Strips for Diabetes
May 3, 2019 - Anti-inflammatory drugs ineffective for prevention of Alzheimer’s disease
May 3, 2019 - Study tracks Pennsylvania’s oil and gas waste-disposal practices
May 3, 2019 - Creating a better radiation diagnostic test for astronauts
May 3, 2019 - Vegans are often deficient in these four nutrients
May 3, 2019 - PPDC announces seed grants to develop medical devices for children
May 3, 2019 - Study maps out the frequency and impact of water polo head injuries
May 3, 2019 - Research on Reddit identifies risks associated with unproven treatments for opioid addiction
May 3, 2019 - Good smells may help ease tobacco cravings
May 3, 2019 - Medical financial hardship found to be very common among people in the United States
May 3, 2019 - Researchers develop multimodal system for personalized post-stroke rehabilitation
May 3, 2019 - Study shows significant mortality benefit with CABG over percutaneous coronary intervention
May 3, 2019 - Will gene-editing of human embryos ever be justifiable?
May 3, 2019 - FDA Approves Dengvaxia (dengue vaccine) for the Prevention of Dengue Disease in Endemic Regions
May 3, 2019 - Why Tonsillitis Keeps Coming Back
May 3, 2019 - Fighting the opioid epidemic with data
May 3, 2019 - Maggot sausages may soon be a reality
May 3, 2019 - Deletion of ATDC gene prevents development of pancreatic cancer in mice
May 2, 2019 - Targeted Therapy Promising for Rare Hematologic Cancer
May 2, 2019 - Alzheimer’s disease is a ‘double-prion disorder,’ study shows
May 2, 2019 - Reservoir bugs: How one bacterial menace makes its home in the human stomach
May 2, 2019 - Clinical, Admin Staff From Cardiology Get Sneak Peek at Epic
May 2, 2019 - Depression increases hospital use and mortality in children
May 2, 2019 - Vicon and NOC support CURE International to create first gait lab in Ethiopia
May 2, 2019 - Researchers use 3D printer to make paper organs
May 2, 2019 - Viral infection in utero associated with behavioral abnormalities in offspring
May 2, 2019 - U.S. Teen Opioid Deaths Soaring
May 2, 2019 - Opioid distribution data should be public
May 2, 2019 - In the Spotlight: “I’m learning every single day”
May 2, 2019 - 2019 Schaefer Scholars Announced
May 2, 2019 - Podcast: KHN’s ‘What The Health?’ Bye-Bye, ACA, And Hello ‘Medicare-For-All’?
May 2, 2019 - Study describes new viral molecular evasion mechanism used by cytomegalovirus
May 2, 2019 - SLU study suggests a more equitable way for Medicare reimbursement
May 2, 2019 - Scientists discover first gene involved in lower urinary tract obstruction
May 2, 2019 - Researchers identify 34 genes associated with increased risk of ovarian cancer
May 2, 2019 - Many low-income infants receive formula in the first few days of life, finds study
May 2, 2019 - Global study finds high success rate for hip and knee replacements
May 2, 2019 - Taking depression seriously: What is it?
May 2, 2019 - With Head Injuries Mounting, Will Cities Put Their Feet Down On E-Scooters?
May 2, 2019 - Scientists develop small fluorophores for tracking metabolites in living cells
May 2, 2019 - Study casts new light into how mothers’ and babies’ genes influence birth weight
May 2, 2019 - Researchers uncover new brain mechanisms regulating body weight
May 2, 2019 - Organ-on-chip systems offered to Asia-Pacific regions by Sydney’s AXT
May 2, 2019 - Adoption of new rules drops readmission penalties against safety net hospitals
May 2, 2019 - Kids and teens who consume zero-calorie sweetened beverages do not save calories
May 2, 2019 - Improved procedure for cancer-related erectile dysfunction
May 2, 2019 - Hormone may improve social behavior in autism
May 2, 2019 - Alzheimer’s disease may be caused by infectious proteins called prions
May 2, 2019 - Even Doctors Can’t Navigate Our ‘Broken Health Care System’
May 2, 2019 - Study looks at the impact on criminal persistence of head injuries
May 2, 2019 - Honey ‘as high in sugars as table sugar’
May 2, 2019 - Innovations to U.S. food system could help consumers in choosing healthy foods
May 2, 2019 - FDA Approves Mavyret (glecaprevir and pibrentasvir) as First Treatment for All Genotypes of Hepatitis C in Pediatric Patients
May 2, 2019 - Women underreport prevalence and intensity of their own snoring
May 2, 2019 - Concussion summit focuses on science behind brain injury
May 2, 2019 - Booker’s Argument For Environmental Justice Stays Within The Lines
May 2, 2019 - Cornell research explains increased metastatic cancer risk in diabetics
May 2, 2019 - Mount Sinai study provides fresh insights into cellular pathways that cause cancer
May 2, 2019 - Researchers to study link between prenatal pesticide exposures and childhood ADHD
May 2, 2019 - CoGEN Congress 2019: Speakers’ overviews
May 2, 2019 - A new strategy for managing diabetic macular edema in people with good vision
May 2, 2019 - Sagent Pharmaceuticals Issues Voluntary Nationwide Recall of Ketorolac Tromethamine Injection, USP, 60mg/2mL (30mg per mL) Due to Lack of Sterility Assurance
May 2, 2019 - Screen time associated with behavioral problems in preschoolers
May 2, 2019 - Hormone reduces social impairment in kids with autism | News Center
May 2, 2019 - Researchers synthesize peroxidase-mimicking nanozyme with low cost and superior catalytic activity
May 2, 2019 - Study results of a potential drug to treat Type 2 diabetes in children announced
May 2, 2019 - Multigene test helps doctors to make effective treatment decisions for breast cancer patients
May 2, 2019 - UNC School of Medicine initiative providing unique care to dementia patients
May 2, 2019 - Nestlé Health Science and VHP join forces to launch innovative COPES program for cancer patients
May 2, 2019 - Study examines how our brain generates consciousness and loses it during anesthesia
May 2, 2019 - Transition Support Program May Aid Young Adults With Type 1 Diabetes
May 2, 2019 - Study shows how neutrophils exacerbate atherosclerosis by inducing smooth muscle-cell death
May 2, 2019 - Research reveals complexity of how we make decisions
Forming 3D Neuronal Models of the Brain

Forming 3D Neuronal Models of the Brain

An interview with Dr Grischa Chandy, conducted at SfN 2018 by Alina Shrourou, BSc.

How can 3D imaging and analysis improve our understanding of the brain?

The ability to acquire and analyze 3D dimensions provides a more physiologically relevant model to study. With 3D imaging and analysis, researchers can study physiology and pathophysiology in the 3D context. For example, instead of a sample with just neurons, it can be a 3D sample with neurons and astrocytes, enabling researchers to gain a greater understanding of neurodegenerative disease and discover potential therapeutics.

Neuron overlay taken at 60x on the ImageXpress Micro Confocal system

What is a 3D neuronal model and how can these be used to develop potential therapeutics?

With induced pluripotent stem cell (iPSC) technologies, it is possible to generate 3D models from individual patients and correlate the patient’s genome with phenotype and functional response to potential drug candidates. StemoniX®, one of the companies we collaborate with, has developed a 3D neural assay model for high-content, high-throughput screening.

Confocal image of a StemoniX microBrain® 3D spheroid showing the presence of neurons (MAP2, green) and astrocytes (GFAP, red).  Nuclei are stained blue (DAPI). Credit: StemoniX

Are there any essential factors or abilities needed in a device in order to achieve a clear 3D image? If so, what are these?

Molecular Devices specializes in automating and scaling workflows. It is critical to have a system flexible enough and capable of finding and focusing on a variety of 3D sample holders that researchers are using like hydrogels or microplates with ultra-low attachment, round bottom wells. To properly acquire the image, the system needs to be able to automatically focus on and then, optically segment the 3D model.

The system also needs to be able to analyze the 3D dataset in 3-dimensional space, in order to interconnect objects that span multiple serial optical sections. And, if physiological measurements need to be made (example of intracellular calcium changes or membrane potential), the system needs to have fast sensitivity and image streaming capabilities (ultra-fast >60 frames per second).

3D visualization of cell nuclei and TUJ-positive neurites and cell bodies. Credit: Ectica and Oksana Sirenko, Molecular Devices

What 3D imaging systems do Molecular Devices provide to help scientists with their neuroscience research? What 3D imaging system are you promoting at Neuroscience 2018?

Our ImageXpress® Micro Confocal High-Content Imaging System features a spinning disk confocal with a unique dual-disk offering and a sCMOS camera that offers sensitive, high-speed imaging and a large field of view. The system offers the ability to design custom image analysis modules that include true 3D analysis with fast multi-CPU processing of large 3D datasets.

We also offer configurable environmental control options that are automation-friendly, for multi-day experiments. Environmental control is integrated within the system and we also have the capability to automate between an incubator and the imager.

We have an Advanced Workflow and Engineered Solutions team to help researchers looking to scale or to automate their system. For example, the system can be automated to expand a study to >44 plates in live imaging mode and/or researchers can run CO2 control and hypoxic experiments.

The ImageXpress® Micro Confocal High-Content Imaging System  

Please give an overview of the technology behind this product(s) which allows such accurate 3D cell representation to be achieved.

The ability to acquire and analyze 3D datasets is driven by our unique confocal design and our tools that allow you to scale 3D image analysis across a plate. However, as people explore what and how to create 3D models, researchers will need to rely on more than that. Researchers need a method to automate the collection of data in live cells, in addition to a multitude of variable conditions. This would be different from a basic research workflow.

We provide a few different workflows to counter this problem. One is our unique live cell incubation system that enables time lapse imaging experiments for extended periods. Another is ensuring our system is designed to work with external lab automation, which makes it possible to expand studies across multiple plates and to enable the study of more conditions and compounds. The ImageXpress Micro Confocal system makes it possible to monitor stem cell growth, automate cell culture, and monitor for key stem cell differentiation checkpoints prior to running endpoint assays.

What are the applications of Molecular Devices’ 3D imaging solutions?

Stem cell development, 3D cell culture, organ-on-a-chip data acquisition, 3D screening, StemoniX microBrain® 3D, imaging of iPSCs in ultra-low attachment, round bottom well spheroid plates, physiology experiments with real-time pipetting, high-speed, co-culturing, immuno-oncology studies, t-cell mediated cell death, and more.

What do you hope to gain from Neuroscience 2018?

In our discussion with researchers and other industry experts, we hope to gain further insight into the future direction in neuroscience research. These discussions help us to develop new, innovative tools to meet the needs of the research community.

About Molecular Devices

Molecular Devices provide customers with innovative bioanalytical solutions for protein and cell biology in life science research, pharmaceutical and biotherapeutic development.

With over 140,000 placements in laboratories around the world, Molecular Devices instruments have contributed to remarkable scientific research described in over 150,000 peer reviewed publications. Included within a broad product portfolio are platforms for high-throughput screening, genomic and cellular analysis, colony selection and microplate detection.

Tagged with:

About author

Related Articles