Breaking News
December 17, 2018 - How the Mediterranean Diet Can Help Women’s Hearts
December 17, 2018 - Sustained connections associated with symptoms of autism
December 17, 2018 - Concussion rates among young football players were higher than previously reported
December 17, 2018 - Cresco Labs granted approval to operate marijuana dispensary in Ohio
December 17, 2018 - Study provides insight into health risks facing new mothers
December 17, 2018 - AMSBIO expands Wnt signaling pathway product range to aid research
December 16, 2018 - Surgical treatment unnecessary for many prostate cancer patients
December 16, 2018 - Excess weight responsible for cancers globally finds report
December 16, 2018 - Regular sex associated with greater enjoyment of life in seniors
December 16, 2018 - Social stigma contributes to poor mental health in the autistic community
December 16, 2018 - Multidisciplinary team successfully performs complex surgery on patient suffering from enlarged skull
December 16, 2018 - Experts analyze data that can guide antidepressant discontinuation
December 16, 2018 - Menlo Therapeutics’ Successful Phase 2 Clinical Trial of Serlopitant Demonstrates Reduction of Pruritus Associated with Psoriasis
December 16, 2018 - Siblings of children with autism or ADHD are at elevated risk for both disorders
December 16, 2018 - New project aims to understand why and how metabolic disorders develop in patients
December 16, 2018 - Diets containing GM maize have no harmful effects on health or metabolism of rats
December 16, 2018 - Are doctors and teachers confusing immaturity and attention deficit?
December 16, 2018 - Hearing loss linked with increased risk for premature death
December 16, 2018 - Chromatrap buffer reagents for lysing cells offer many benefits
December 16, 2018 - Young Breast Cancer Patients Face Higher Risk for Osteoporosis
December 16, 2018 - 3-D printing offers helping hand to people with arthritis
December 16, 2018 - Community Health Choice helps manage complex and chronic care conditions
December 16, 2018 - Regular trips out could dramatically reduce depression in older age
December 16, 2018 - CWRU to use VivaLNK’s Vital Scout device for stress study in student athletes
December 16, 2018 - ‘Easy Way Out’? Stigma May Keep Many From Weight Loss Surgery
December 16, 2018 - Gout drug may protect against chronic kidney disease
December 16, 2018 - Talking about memories enhances the wellbeing of older and younger people
December 16, 2018 - Occupational exposure to pesticides increases risk for cardiovascular disease among Latinos
December 16, 2018 - A biomarker in the brain’s circulation system may be Alzheimer’s earliest warning
December 16, 2018 - Magnesium may play important role in optimizing vitamin D levels, study shows
December 16, 2018 - The effect of probiotics on intestinal flora of premature babies
December 16, 2018 - Parents spend more time talking with kids about mechanics of using mobile devices
December 16, 2018 - Biohaven Announces Positive Results from Ongoing Rimegepant Long-Term Safety Study
December 16, 2018 - Arterial stiffness may predict dementia risk
December 16, 2018 - Study explores link between work stress and increased cancer risk
December 16, 2018 - Sex work criminalization linked to incidences of violence finds study
December 16, 2018 - Johns Hopkins researchers discover swarming behavior in fish-dwelling parasite
December 16, 2018 - Schistosomiasis prevention and treatment could help control HIV
December 16, 2018 - Early postpartum opioids linked with persistent usage
December 16, 2018 - Johns Hopkins researchers identify molecular causes of necrotizing enterocolitis in preemies
December 16, 2018 - Advanced illumination expands capabilities of light-sheet microscopy
December 16, 2018 - Alzheimer’s could possibly be spread via contaminated neurosurgery
December 16, 2018 - Unraveling the complexity of cancer biology can prompt new avenues for drug development
December 16, 2018 - Inflammatory Bowel Disease, Prostate Cancer Linked
December 16, 2018 - Cannabis youth prevention strategy should target mental wellbeing
December 15, 2018 - Recent developments and challenges in hMAT inhibitors
December 15, 2018 - Sewage bacteria found lurking in Hudson River sediments
December 15, 2018 - CDC selects UMass Amherst biostatistician model that helps predict influenza outbreaks
December 15, 2018 - Researchers reveal brain mechanism that drives itch-evoked scratching behavior
December 15, 2018 - New computer model helps predict course of the disease in prostate cancer patients
December 15, 2018 - Obesity to Blame for Almost 1 in 25 Cancers Worldwide
December 15, 2018 - How the brain tells you to scratch that itch
December 15, 2018 - New findings could help develop new immunotherapies against cancer
December 15, 2018 - World’s largest AI-powered medical research network launched by OWKIN
December 15, 2018 - Young people suffering chronic pain battle isolation and stigma as they struggle to forge their identities
December 15, 2018 - Lifespan extension at low temperatures depends on individual’s genes, study shows
December 15, 2018 - New ingestible capsule can be controlled using Bluetooth wireless technology
December 15, 2018 - Researchers uncover microRNAs involved in the control of social behavior
December 15, 2018 - Research offers hope for patients with serious bone marrow cancer
December 15, 2018 - Link between poverty and obesity is only about 30 years old, study shows
December 15, 2018 - Mass spectrometry throws light on old case of intentional heavy metal poisoning
December 15, 2018 - BeyondSpring Announces Phase 3 Study 105 of its Lead Asset Plinabulin for Chemotherapy-Induced Neutropenia Meets Primary Endpoint at Interim Analysis
December 15, 2018 - Study finds that in treating obesity, one size does not fit all
December 15, 2018 - Tenacity and flexibility help maintain psychological well-being, mobility in older people
December 15, 2018 - Study reveals role of brain mechanism in memory recall
December 15, 2018 - High levels of oxygen encourage the brain to remain in deep, restorative sleep
December 15, 2018 - Experimental HIV vaccine strategy works in non-human primates, research shows
December 15, 2018 - Genetically modified pigs could limit replication of classical swine fever virus, study shows
December 15, 2018 - FDA Approves Herzuma (trastuzumab-pkrb), a Biosimilar to Herceptin
December 15, 2018 - Cost and weight-loss potential matter most to bariatric surgery patients
December 15, 2018 - Cancer Research UK and AstraZeneca open new Functional Genomics Centre
December 15, 2018 - New research lays out potential path for treatment of Huntington’s disease
December 15, 2018 - Prestigious R&D 100 Award presented to Leica Microsystems
December 15, 2018 - Study shows septin proteins detect and kill gut pathogen, Shigella
December 15, 2018 - Study sheds new light on disease-spreading mosquitoes
December 15, 2018 - 2017 Saw Slowing in National Health Care Spending
December 15, 2018 - Monitoring movement reflects efficacy of mandibular splint
December 15, 2018 - Study supports BMI as useful tool for assessing obesity and health
December 15, 2018 - Self-guided, internet-based therapy platforms effectively reduce depression
December 15, 2018 - Organically farmed food has bigger climate impact than conventional food production
University of Wuerzburg implements new turnkey confocal imaging system

University of Wuerzburg implements new turnkey confocal imaging system

image_pdfDownload PDFimage_print

Technology innovators in super-resolution microscopy, Amsterdam-based Confocal.nl,reports on the implementation of a new turnkey confocal imaging system in the laboratory of Professor Markus Sauer at the University of Würzburg.

Andreas Kurz with the RCM confocal/dSTORM system located in Professor Markus Sauer’s laboratory at the University of Würzburg

Professor Markus Saueris the Chair of the group for biotechnology and biophysics at the University of Würzburg. He is very interested in imaging: “Imaging technologies are central platforms that drive fundamental research in virtually all disciplines across the biological and medical sciences. By seeing how a system looks, functions can be understood and comparison made between healthy and unhealthy systems such as cells, tissue, a whole organism or population, plants, animals and humans.”

Professor Sauer’s PhD student, Andreas Kurz, describes how he implemented a new technique called Re-scan Confocal Microscopy (RCM):

We integrated the RCM module from Confocal.nl to our existing single molecule dSTORM setup to establish a new correlative imaging technique. As RCM is based on image scanning, it intrinsically has super-resolution capabilities (170 nm) combined with the advantages of a conventional confocal microscope. Now with this combined correlative imaging setup, a new variety of experiments are possible. Recording dynamic processes and the long-term observation of cell activity followed by resolving the structure of interest, a superb resolution of around 20 nm will now be possible.”

Mr Kurz continues to describe the process to select RCM:

Confocal microscopy is an imaging technique with lots of advantages. First of all, it is an easy-to-use tool for routine work in the field of fluorescence. Now with RCM, we have an enhanced version of confocal microscopy which allows the observation of sensitive samples and more complex imaging procedures at a higher resolution than other confocal microscopies. This system is now used to complement other techniques such as structured illumination microscopy, single molecule localization microscopy setups and light sheet illumination microscopy.

We ordered our RCM unit from Confocal.nl. Their CTO, Erik Manders, visited to personally assist with the installation and training. Since then, we have had excellent support with updates through software plugins and exchanging applications experiences. Concerning the setup, we had the microscope body already installed (Nikon Eclipse TiE). In addition, we purchased a Skyra multi-line laser unit from Cobolt and an Andor Zyla 4.2 sCMOS camera. It was great to be able to use our existing microscope and then add specific components to, in effect, produce a turnkey set-up to match our demanding needs. Confocal.nl continue to provide a lot of support (local as well as via mail/phone). Having an accessible and manageable team, we get the ease of communication with a personal touch, something I really prefer in this environment.”

Concluding, Mr Kurz says:

Now, our experimental set-up is a unique, fast-scanning, resolution-enhanced and live-cell capable system configured for both dSTORM and RCM that delivers super-resolution performance. Our main reasons for purchase have been realized in terms of performance of the complete package integrating our existing components at a very fair price. We have found the multi-color capability with incredibly low laser power very useful for live cell imaging.”

Speaking about the installation at Würzburg, Confocal.nl’s CEO, Peter Drent, says;

We were extremely pleased to have Professor Sauer’s team select the RCM. They provided precise requirements from which we have been able to configure an excellent, working system with high resolution and sensitivity. While dSTORM is quite an intensive experimental technique involving complex biological and chemical sample preparation, it does provide 20 nm resolution. This is now complemented by RCM also giving super-resolution at 170 nm with minimal sample preparation delivering a simple, instant image.”

Source:

The Sauer Laboratory successfully implements Re-scan Confocal Microscopy with turnkey system from Confocal.nl 

Tagged with:

About author

Related Articles