Breaking News
May 3, 2019 - Vaping and Smoking May Signal Greater Motivation to Quit
May 3, 2019 - Dementia looks different in brains of Hispanics
May 3, 2019 - Short-Staffed Nursing Homes See Drop In Medicare Ratings
May 3, 2019 - Study of teens with eating disorders explores how substance users differ from non-substance users
May 3, 2019 - Scientists develop new video game that may help in the study of Alzheimer’s
May 3, 2019 - Arc Bio introduces Galileo Pathogen Solution product line at ASM Clinical Virology Symposium
May 3, 2019 - Cornell University study uncovers relationship between starch digestion gene and gut bacteria
May 3, 2019 - How to Safely Use Glucose Meters and Test Strips for Diabetes
May 3, 2019 - Anti-inflammatory drugs ineffective for prevention of Alzheimer’s disease
May 3, 2019 - Study tracks Pennsylvania’s oil and gas waste-disposal practices
May 3, 2019 - Creating a better radiation diagnostic test for astronauts
May 3, 2019 - Vegans are often deficient in these four nutrients
May 3, 2019 - PPDC announces seed grants to develop medical devices for children
May 3, 2019 - Study maps out the frequency and impact of water polo head injuries
May 3, 2019 - Research on Reddit identifies risks associated with unproven treatments for opioid addiction
May 3, 2019 - Good smells may help ease tobacco cravings
May 3, 2019 - Medical financial hardship found to be very common among people in the United States
May 3, 2019 - Researchers develop multimodal system for personalized post-stroke rehabilitation
May 3, 2019 - Study shows significant mortality benefit with CABG over percutaneous coronary intervention
May 3, 2019 - Will gene-editing of human embryos ever be justifiable?
May 3, 2019 - FDA Approves Dengvaxia (dengue vaccine) for the Prevention of Dengue Disease in Endemic Regions
May 3, 2019 - Why Tonsillitis Keeps Coming Back
May 3, 2019 - Fighting the opioid epidemic with data
May 3, 2019 - Maggot sausages may soon be a reality
May 3, 2019 - Deletion of ATDC gene prevents development of pancreatic cancer in mice
May 2, 2019 - Targeted Therapy Promising for Rare Hematologic Cancer
May 2, 2019 - Alzheimer’s disease is a ‘double-prion disorder,’ study shows
May 2, 2019 - Reservoir bugs: How one bacterial menace makes its home in the human stomach
May 2, 2019 - Clinical, Admin Staff From Cardiology Get Sneak Peek at Epic
May 2, 2019 - Depression increases hospital use and mortality in children
May 2, 2019 - Vicon and NOC support CURE International to create first gait lab in Ethiopia
May 2, 2019 - Researchers use 3D printer to make paper organs
May 2, 2019 - Viral infection in utero associated with behavioral abnormalities in offspring
May 2, 2019 - U.S. Teen Opioid Deaths Soaring
May 2, 2019 - Opioid distribution data should be public
May 2, 2019 - In the Spotlight: “I’m learning every single day”
May 2, 2019 - 2019 Schaefer Scholars Announced
May 2, 2019 - Podcast: KHN’s ‘What The Health?’ Bye-Bye, ACA, And Hello ‘Medicare-For-All’?
May 2, 2019 - Study describes new viral molecular evasion mechanism used by cytomegalovirus
May 2, 2019 - SLU study suggests a more equitable way for Medicare reimbursement
May 2, 2019 - Scientists discover first gene involved in lower urinary tract obstruction
May 2, 2019 - Researchers identify 34 genes associated with increased risk of ovarian cancer
May 2, 2019 - Many low-income infants receive formula in the first few days of life, finds study
May 2, 2019 - Global study finds high success rate for hip and knee replacements
May 2, 2019 - Taking depression seriously: What is it?
May 2, 2019 - With Head Injuries Mounting, Will Cities Put Their Feet Down On E-Scooters?
May 2, 2019 - Scientists develop small fluorophores for tracking metabolites in living cells
May 2, 2019 - Study casts new light into how mothers’ and babies’ genes influence birth weight
May 2, 2019 - Researchers uncover new brain mechanisms regulating body weight
May 2, 2019 - Organ-on-chip systems offered to Asia-Pacific regions by Sydney’s AXT
May 2, 2019 - Adoption of new rules drops readmission penalties against safety net hospitals
May 2, 2019 - Kids and teens who consume zero-calorie sweetened beverages do not save calories
May 2, 2019 - Improved procedure for cancer-related erectile dysfunction
May 2, 2019 - Hormone may improve social behavior in autism
May 2, 2019 - Alzheimer’s disease may be caused by infectious proteins called prions
May 2, 2019 - Even Doctors Can’t Navigate Our ‘Broken Health Care System’
May 2, 2019 - Study looks at the impact on criminal persistence of head injuries
May 2, 2019 - Honey ‘as high in sugars as table sugar’
May 2, 2019 - Innovations to U.S. food system could help consumers in choosing healthy foods
May 2, 2019 - FDA Approves Mavyret (glecaprevir and pibrentasvir) as First Treatment for All Genotypes of Hepatitis C in Pediatric Patients
May 2, 2019 - Women underreport prevalence and intensity of their own snoring
May 2, 2019 - Concussion summit focuses on science behind brain injury
May 2, 2019 - Booker’s Argument For Environmental Justice Stays Within The Lines
May 2, 2019 - Cornell research explains increased metastatic cancer risk in diabetics
May 2, 2019 - Mount Sinai study provides fresh insights into cellular pathways that cause cancer
May 2, 2019 - Researchers to study link between prenatal pesticide exposures and childhood ADHD
May 2, 2019 - CoGEN Congress 2019: Speakers’ overviews
May 2, 2019 - A new strategy for managing diabetic macular edema in people with good vision
May 2, 2019 - Sagent Pharmaceuticals Issues Voluntary Nationwide Recall of Ketorolac Tromethamine Injection, USP, 60mg/2mL (30mg per mL) Due to Lack of Sterility Assurance
May 2, 2019 - Screen time associated with behavioral problems in preschoolers
May 2, 2019 - Hormone reduces social impairment in kids with autism | News Center
May 2, 2019 - Researchers synthesize peroxidase-mimicking nanozyme with low cost and superior catalytic activity
May 2, 2019 - Study results of a potential drug to treat Type 2 diabetes in children announced
May 2, 2019 - Multigene test helps doctors to make effective treatment decisions for breast cancer patients
May 2, 2019 - UNC School of Medicine initiative providing unique care to dementia patients
May 2, 2019 - Nestlé Health Science and VHP join forces to launch innovative COPES program for cancer patients
May 2, 2019 - Study examines how our brain generates consciousness and loses it during anesthesia
May 2, 2019 - Transition Support Program May Aid Young Adults With Type 1 Diabetes
May 2, 2019 - Study shows how neutrophils exacerbate atherosclerosis by inducing smooth muscle-cell death
May 2, 2019 - Research reveals complexity of how we make decisions
New technique uses UV and red light to decontaminate organs before transplantation

New technique uses UV and red light to decontaminate organs before transplantation

A new technique for the decontamination of organs before transplantation using ultraviolet and red light irradiation has been developed by Brazilian and Canadian researchers and is described in an article published in the journal Nature Communications.

The research is supported by São Paulo Research Foundation – FAPESP and has been partially conducted at the Optics and Photonics Research Center (CEPOF), hosted by the University of São Paulo (USP) at São Carlos in São Paulo State (Brazil).

“This biophotonic technique is revolutionary, as it helps avoid the transmission of diseases during organ transplantation,” said Vanderlei Bagnato, Full Professor at the University of São Paulo, Director of its São Carlos Physics Institute (IFSC-USP), and principal investigator for CEPOF, one of the Research, Innovation and Dissemination Centers (RIDCs) supported by FAPESP.

Bagnato’s group partnered with researchers at the University of Toronto in Canada, which has the world’s largest lung transplantation program, having performed 197 such surgeries in 2018 alone. According to the thoracic surgeon Marcelo Cypel, who heads the service, the number of transplants could be higher if organs could be decontaminated, especially when the prospective donor has a chronic viral infection such as hepatitis C.

“Ten patients have so far been tested [using the biophotonic therapy],” Cypel said. “The new technique significantly reduced transplant organ viral load in eight of these patients. The procedure all but eliminated the virus in two others.”

The method described in the article (“Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion”) involves ultraviolet and red light irradiation to reduce viral and bacterial loads in infected organs to prevent the transmission of diseases such as hepatitis to transplant recipients.

In addition to FAPESP, the research was also funded by the Canadian Institutes of Health Research, the Toronto General and Western Hospital Foundation, and Brazil’s National Council for Scientific and Technological Development (CNPq).

According to Bagnato, the technique was initially developed to treat lungs but is being adapted for livers and kidneys. “This should greatly improve postoperative conditions for transplant recipients,” he said. “It will also enable us to use organs that we must currently reject because of the level of contamination.”

Two-step decontamination

Lungs are decontaminated before transplantation by having the blood replaced by a preservation liquid in a procedure known as perfusion that was developed in Canada by Cypel.

“Perfusion reduces the viral and bacterial loads but cannot eliminate them completely. As a result, the patient has to be treated with antibiotics and antivirals for three months after the transplant,” Cypel explained.

“Thinking about ways to further reduce or eliminate the viral load in organs for transplantation, specifically hepatitis C virus, I considered the possibility of using ultraviolet light decontamination methods, which are commonly employed to decontaminate blood, for example. So about four years ago, we began partnering with Bagnato and his team at São Carlos. He and his colleagues came over to visit us and study the technique. Only a month later they sent us the first prototype of the ultraviolet irradiation decontamination machine.”

“The biophotonic decontamination technique developed at our São Carlos laboratory consists of two specific procedures performed concurrently,” said Cristina Kurachi, a professor at IFSC-USP and a participant in the project.

During perfusion, while the researchers make the liquid circulate in the lung to be transplanted, they add molecules to the lung tissue, and biophotonic decontamination takes place directly in the organ through irradiation with red light with a wavelength of 660 nanometers (nm) until photodynamic oxidation eliminates the microorganisms in the tissue.

At the same time, the viral load is flushed away by the circulating liquid, which is continuously decontaminated by ultraviolet irradiation with a wavelength of 254 nm.

“The ultraviolet irradiation directly destroys microorganisms by breaking down the molecules present in bacteria and viruses. The bacteria are killed, and the viruses are completely inactivated. Red light irradiation decontaminates indirectly via photosensitization,” Kurachi said.

This biophotonic therapy involves the introduction of a photosensitizing drug into the perfusion liquid. Activation of the drug requires oxygen molecules (present in viruses) and red light irradiation at a specific wavelength (660 nm). When the photosensitizing drug is bathed in this red light, its molecules absorb energy, which is transferred to the oxygen molecules in the virus, making them highly oxidized. This causes irreversible damage to the membranes and genetic material of several viruses, including hepatitis C virus and HIV-1.

“The perfusion solution is special and very expensive,” Bagnato said. “It’s made in such a way as to preserve the organ. Because of the cost, as little as possible is used in the procedure. Thanks to the technique and equipment we’ve developed, a liter of the perfusate can be flushed through the organ hundreds of times to remove the contaminants completely.”

The method was first tested on human lungs rejected for transplantation to determine whether the tissue viral load could be reduced by irradiation. According to Cypel, the viral load was found to have fallen drastically after the procedure.

“The next step was to subject pig lungs to the same procedure and then transplant them to see if the procedure caused any biochemical or morphological damage to tissue. It did not,” Cypel said.

Finally, the technique was tested on patients. “In the first ten transplants we performed, the new technique eliminated hepatitis C virus from organs donated to two patients. In the other eight patients, viral load fell sharply after surgery but rose again seven days later, and the patients had to be given antiviral treatment for three months,” he said.

“An important finding was that when the virus wasn’t eliminated it reappeared in the patient’s lab tests after seven days. With this information, we’ve since performed two other transplants where antiviral treatment concentrated in the first week after the operation. The virus was eliminated in both cases,” Cypel said.

According to Bagnato, this biophotonic therapy will be refined to assure even sharper reductions in the viral and bacterial loads, increasing the chances of successful transplants. “Our aim is to have light-based therapy eliminate all viral and bacterial contaminants in organs to be transplanted. If so, it may even be possible to do without the perfusate,” he said.

The clinical part of the project is being conducted by Cypel and his group in Toronto. The researchers at IFSC-USP designed the new biophotonic technique, developed the instruments, and are participating in the analysis of the results. In addition to Bagnato and Kurachi, the Brazilian team at IFSC-USP also includes Natalia Inada.

A patent application has been filed in Canada, and two multinationals have expressed an interest in studying the possibility of producing and marketing the equipment. The researchers are now working on the implementation of a liver and kidney decontamination program in Brazil.

“All this has only been possible thanks to the philosophy introduced by FAPESP’s RIDC program, which encouraged us to collaborate internationally and at the same time produce practically relevant knowledge,” Bagnato said.

Tagged with:

About author

Related Articles