Breaking News
December 15, 2017 - Caris Life Sciences reveals identification of new mechanism of action to treat NHL
December 15, 2017 - Loyola Medicine study finds high success rate for diabetic Charcot foot surgery
December 15, 2017 - Bone marrow edema does not increase due to intense physical activity, study finds
December 15, 2017 - Human ‘common cold’ virus kills healthy chimpanzees in Uganda
December 15, 2017 - Experience of reflex walking refines perception of biological motion during early infancy
December 15, 2017 - FDA Approves Admelog (insulin lispro) Rapid-Acting “Follow-On” Insulin Product to Treat Diabetes
December 14, 2017 - Friday Feedback: Good Idea for Ex-Pharma Exec to Run HHS?
December 14, 2017 - More than 200 people sickened onboard Ovation of Seas cruise
December 14, 2017 - FDA announces new approach for sharing updates on antibiotics, antifungal drugs to physicians
December 14, 2017 - Steroid study provides new insights into medicines’ side effects
December 14, 2017 - Government announces plans to include eye test reminder during driving license renewal
December 14, 2017 - Non-ionizing radiation from magnetic fields could have adverse biological impacts on health
December 14, 2017 - Bi-annual MRI beats mammograms in detecting breast cancer among women with genetic risk
December 14, 2017 - Researchers develop new method for quickly detecting signs of multiple sclerosis
December 14, 2017 - In era of increased competition, hospitals fret over ratings
December 14, 2017 - Female veterans experience improvement in low back pain with course of chiropractic care
December 14, 2017 - Relieving Symptoms of Cystic Fibrosis with Exercise
December 14, 2017 - FDA Alert: Blue Pearl All Natural Male Enhancement Supplement: Recall
December 14, 2017 - CardioBrief: In Defense Of ORBITA
December 14, 2017 - Definition of High Blood Pressure Drops: MedlinePlus Health News
December 14, 2017 - Drug may help surgical patients stop opioids sooner
December 14, 2017 - Researchers develop biosensor that enables development of new health tests
December 14, 2017 - Radiation therapy can be used to treat patients with life-threatening heart rhythm
December 14, 2017 - UVA researchers developing tool to help prostate cancer patients weigh treatment options
December 14, 2017 - Experts tell Congress how to cut drug prices
December 14, 2017 - Researchers use cryptographic techniques to decode activity of motor neurons
December 14, 2017 - Study finds changes in the heart after spinal cord injury
December 14, 2017 - Health Highlights: Dec. 12, 2017
December 14, 2017 - Pelzman’s Picks: How States Can Cut Disparities in Care and Costs
December 14, 2017 - New Hemophilia Treatment Stems Bleeding Episodes: MedlinePlus Health News
December 14, 2017 - Onetime ‘world’s heaviest man’ has second surgery in Mexico
December 14, 2017 - Belgian researchers create transplantable artificial ovary prototype
December 14, 2017 - Using atraumatic needles for lumbar punctures decreases risk of complications
December 14, 2017 - Outpatient total knee replacement surgery linked to higher rates of complications
December 14, 2017 - Social impairments can be corrected by brain stimulation
December 14, 2017 - Studies reveal possibility for memory T cells to serve a dual purpose
December 14, 2017 - Antibody-Drug Conjugate Ups PFS in Untreated Hodgkin’s
December 14, 2017 - Study finds reading information aloud to yourself improves memory
December 14, 2017 - Researchers use RNA nanotechnology to program exosomes for delivering effective cancer therapies
December 14, 2017 - Living Lyme disease bacteria found months after antibiotic treatment
December 14, 2017 - These annual checkups help seniors not only survive but thrive
December 14, 2017 - Study reveals impact of diabetes during pregnancy on baby’s heart
December 14, 2017 - Hydraulic fracturing is harmful to infants health, study states
December 14, 2017 - Huntington’s disease drug clears initial hurdles
December 14, 2017 - TPU researchers create 3D-printed models of children’s hearts
December 14, 2017 - Brain responses of children with inherited dyslexia risk predict their future reading speed
December 14, 2017 - People diagnosed with schizophrenia or bipolar disorder may actually have treatable condition
December 14, 2017 - Study: New Furosemide Formulation Simplifies Administration for HF
December 14, 2017 - Discrimination harms your health—and your partner’s
December 14, 2017 - Having older brothers may increase the likelihood of being gay
December 14, 2017 - New scientific yardstick released to help early detection of Alzheimer’s disease
December 14, 2017 - New finding demonstrates what happens at cellular level during onset of type2 diabetes
December 14, 2017 - Study identifies potassium as key to circadian rhythms in red blood cells
December 14, 2017 - Good friends might be your best brain booster as you age
December 14, 2017 - NIH expected to award up to $70 million to launch Alzheimer’s Clinical Trials Consortium
December 14, 2017 - Pitting pathogens against each other could prevent drug resistance emerging
December 14, 2017 - Study provides new insights into development of Sonic Hedgehog medulloblastoma
December 14, 2017 - Dr. Reddy’s Announces Approval of Impoyz (clobetasol propionate) Cream for Plaque Psoriasis
December 14, 2017 - Gene Screens Can Alter Perception, Behavior
December 14, 2017 - Can Scrotal Vein Condition Hike Heart Risks?: MedlinePlus Health News
December 14, 2017 - Molecules in spit may be able to diagnose and predict length of concussions
December 14, 2017 - Children’s Colorado and RxRevu partner to help prescribers better meet needs of pediatric patients
December 14, 2017 - Researchers discover new way to attack drug-resistant prostate cancer cells
December 14, 2017 - Scientists develop new, high resolution method for identifying microbial species and strains
December 14, 2017 - Declining trend of salmonellosis cases has leveled off in the EU
December 14, 2017 - Death receptors in the blood can help measure risk of cardiovascular diseases, type 2 diabetes
December 14, 2017 - How to Perk Up the Holidays for Hospital Patients
December 14, 2017 - Prolonged Sedation May be Bad for Baby’s Brain
December 14, 2017 - The pediatric submersion score predicts children at low risk for injury following submersions
December 14, 2017 - Video game helps doctors to quickly recognize trauma patients who need high levels of care
December 14, 2017 - Younger persons newly-diagnosed with type 2 diabetes have poorer health than older patients
December 14, 2017 - Clinician re-examines evidence on re-use of catheters and UTIs in people with spinal cord injuries
December 14, 2017 - UK and Russian researchers join forces against AMR
December 14, 2017 - Results of Bariatric Surgery Hold Up Over Time
December 14, 2017 - High-intensity exercise delays Parkinson’s progression
December 14, 2017 - Protein structure could pave way for effective drugs to treat cystic fibrosis
December 14, 2017 - Minority people less likely to see dermatologist for psoriasis treatment
December 14, 2017 - Study indicates decline in use of chemotherapy for early stage breast cancer patients
December 14, 2017 - Chagas disease presents real public health problem to Canadians
December 14, 2017 - Experts call for rigorous clinical trials in use of experimental fetal therapy
Researchers find way to switch tumor cells between 2D and 3D morphology

Researchers find way to switch tumor cells between 2D and 3D morphology

image_pdfDownload PDFimage_print

Paving the way for testing experimental drugs in more realistic environments, scientists at the National Institute of Standards and Technology (NIST) have discovered how to make tiny colonies of cells grow in useful new ways inside petri dishes.

The research team’s discoveries might help designers of miniature “lab-on-a-chip” technologies to grow three-dimensional colonies of liver cancer cells inside a chip’s tiny chambers, rather than the merely two-dimensional colonies that they generally can culture now. Since many solid-tissue tumors are themselves three-dimensional, 3-D cell arrays could furnish more realistic biological environments for testing pharmaceuticals than those that are currently available.

“As tumor cell lines are commonly used for testing anti-cancer compounds, the biomedical community is actively looking for ways to test these drugs in 3-D cell cultures,” said Darwin Reyes-Hernandez, a biomedical engineer at NIST. “Our findings could help bridge the gap between analyses of cells in the lab and in living creatures, a gap that currently limits the drug discovery processes.”

To fulfill the promise of lab-on-a-chip technology, the chip’s interior needs to have many features in common with the body itself, such as many different cell types growing in each other’s presence. Scientists can already explore what a single cell type would do in the presence of a drug molecule simply by growing them together in a laboratory petri dish. But drugs must work in the body, not just a lab experiment. To study cell-cell interactions in a controlled fashion, scientists grow multiple cell types in the dish, making each type grow in a different location by changing the characteristics of the growing surface, a technique called micropatterning.

The NIST team, whose members specialize in the microfluidic technologies that would form much of the lab on a chip’s physical environment, initially had the goal of making two different types of human cells grow side by side on a surface: liver cancer cells as well as endothelial cells, which line blood vessels in the body and are critical for cancer progression. Merely finding a way to create this shared boundary between two cell types would have been a worthy accomplishment, according to team member Kiran Bhadriraju, a NIST guest researcher who is visiting from Theiss Research in La Jolla, California. Existing technologies to create such micropatterned co-cultures are cumbersome, he said, and not easily used in large-scale pharmaceutical testing.

The team theorized that when they coated the surface with two different adhesives–fibronectin alone and a composite of fibronectin and other substances called hybrid cell adhesive material (hCAM)–the liver cancer cells would readily stick only to the hCAM, while the endothelial cells would adhere to the fibronectin. Preliminary experiments validated their hunch, and the discovery provided the NIST scientists with a way to create co-cultures of the tumor and endothelial cells where they wanted them.

Creating the shared boundary they’d initially sought was an accomplishment on its own, but there was more to come. When they took images of the cells using a technique known as laser confocal microscopy, the team also discovered that the cells on the hCAM surface had grown layered arrays in three dimensions. Adding a third protein called transglutaminase–a sticky enzyme that glues protein molecules together–they could make the liver cancer cells instead form arrays only a single cell thick, giving them control over the process.

Knowing this relatively simple relationship among the chemicals, surface and liver cancer cells could be useful for culturing cancer cells together with completely different cell types, he says, and might allow these small cell cultures to be scaled up for the sort of high-throughput work a drug company would need to test large numbers of drug candidates.

“We expect that other cancer cell lines can be used for micropatterning similar co-cultures,” said Bhadriraju. “While the liver cancer cell line used here is an important cell line for the pharmaceutical industry for testing anti-cancer drugs, we haven’t tested yet whether other cancer cell types will form the same types of 3-D structures. But we’re optimistic, as these proteins we coated the surface with are commonly used with other kinds of cancer cells.”

Source:

https://www.nist.gov/news-events/news/2017/11/nist-scientists-discover-how-switch-liver-cancer-cell-growth-2-d-3-d

Tagged with:

About author

Related Articles