Breaking News
December 13, 2018 - Paternal grandfather’s access to food associated with grandson’s mortality risk
December 13, 2018 - Our brain senses angry voices in a flash, study shows
December 13, 2018 - PM2.5 Exposure Linked to Asthma Rescue Medication Use
December 13, 2018 - Can’t exercise? A hot bath may help improve inflammation, metabolism, study suggests
December 13, 2018 - Can artificial intelligence help doctors with the human side of medicine?
December 13, 2018 - Virginia Tech and UC San Diego researchers team up to develop nonopioid drug for chronic pain
December 13, 2018 - NIH offers support for HIV care and prevention research in the southern United States
December 12, 2018 - Activating brain region could revive the urge to socialize among opioid addicts
December 12, 2018 - Relationship impairment appears to interfere with seeking mental health treatment in men
December 12, 2018 - Sleep, Don’t Cram, Before Finals for Better Grades
December 12, 2018 - Effective treatments for urticarial vasculitis
December 12, 2018 - Gun violence is a public health issue: One physician’s story
December 12, 2018 - The Science of Healthy Aging
December 12, 2018 - Yes to yoghurt and cheese: New improved Mediterranean diet
December 12, 2018 - Researchers uncover a number of previously unknown insecticide resistance mechanisms
December 12, 2018 - Regulating the immune system’s ‘regulator’
December 12, 2018 - In breaking bad news, the comfort of silence
December 12, 2018 - Study finds upward link between alcohol consumption and physical activity in college students
December 12, 2018 - FDA issues warning letter to Zhejiang Huahai Pharmaceutical involved in valsartan recall
December 12, 2018 - Presence of antiphospholipid antibodies tied to first-time MI
December 12, 2018 - DNA analysis finds that stethoscopes are teaming with bacteria
December 12, 2018 - New study could help inform research on preventing falls
December 12, 2018 - Women and men with heart attack symptoms receive different care from EMS
December 12, 2018 - Disrupted biological clock can contribute to onset of diseases, USC study shows
December 12, 2018 - New publications generate controversy over the value of reducing salt consumption in populations
December 12, 2018 - New data from TAILORx trial confirms lack of chemo benefit regardless of race or ethnicity
December 12, 2018 - Specific class of biomarkers can accurately indicate the severity of cancer
December 12, 2018 - Meds Taken Do Not Vary With ADL Impairment in Heart Failure
December 12, 2018 - Long-term study shows that HIV-2 is deadlier than previously thought
December 12, 2018 - People living near oil and gas wells show early signs of cardiovascular disease
December 12, 2018 - IONTAS founder and pioneer in phage display technology attends Nobel Prize Award Ceremony
December 12, 2018 - People who eat red meat have high levels of chemical associated with heart disease, study finds
December 12, 2018 - New method uses water molecules to unlock neurons’ secrets
December 12, 2018 - Genetics study offers hope for new acne treatment
December 12, 2018 - New computer model predicts prostate cancer progression
December 12, 2018 - Nobel Laureates lecture about immune checkpoint therapy for cancer treatment
December 12, 2018 - More Illnesses From Tainted Romaine Lettuce Reported
December 12, 2018 - Aspirin could reduce HIV infections in women
December 12, 2018 - The EORTC Brain Tumor Group and Protagen AG collaborate to study immuno-competence of long-term glioblastoma survivors
December 12, 2018 - Insights into magnetotactic bacteria could guide development of biological nanorobots
December 12, 2018 - Sacrificial immune cells alert body to infection
December 12, 2018 - Low-salt diet may be more beneficial for females than males
December 12, 2018 - Major soil organic matter compound battles chronic wasting disease
December 12, 2018 - Findings may open up new ways to treat dwarfism and other ER-stress-related conditions
December 12, 2018 - New computational model provides clearer picture of shape-changing cells’ structure and mechanics
December 12, 2018 - 10 Facts on Patient Safety
December 12, 2018 - Poorest dying nearly 10 years younger than the rich in ‘deeply worrying’ trend for UK
December 12, 2018 - Innovative care model for children with ASD reduces use of behavioral drugs in ED
December 12, 2018 - Spending time in and around Hong Kong’s waters linked to better health and wellbeing
December 12, 2018 - Simple measures to prevent weight gain over Christmas
December 12, 2018 - Research advances offer hope for patient-tailored AML treatment
December 12, 2018 - Researchers discover a ‘blind spot’ in atomic force microscopy
December 12, 2018 - Sprayable gel could help prevent recurrences of cancer after surgery
December 12, 2018 - SLU researchers explore how fetal exposure to inflammation can alter immunity in newborns
December 12, 2018 - How do patients want to discuss symptoms with clinicians?
December 12, 2018 - Zinc chelation may be able to deliver drug to insulin-producing cells
December 12, 2018 - Brigham researchers develop automated, low-cost tool to predict a woman’s ovulation
December 12, 2018 - Some people with Type 2 diabetes may be testing their blood sugar more often than needed
December 12, 2018 - Slow-growing type of glioma may be vulnerable to immunotherapy, suggests study
December 12, 2018 - Study provides new information regarding microRNA function in cellular homeostasis of zebrafish
December 12, 2018 - Study provides new understanding of mysterious ‘hereditary swelling’
December 12, 2018 - Researchers shed new light on how to combat Shiga and ricin toxins
December 12, 2018 - Pregnant Women Commonly Refuse Vaccines
December 12, 2018 - Drug treatment could offer new hope for some patients with brain bleeding
December 12, 2018 - Health care financial burden of animal-related injuries is growing, study says
December 12, 2018 - Macrophage cells could help repair the heart following a heart attack, study finds
December 12, 2018 - Researchers develop new system for efficiently producing human norovirus
December 12, 2018 - New artificial intelligence-based system to differentiate between different types of cancer cells
December 11, 2018 - Brazilian professors propose guidelines for therapeutic use of melatonin
December 11, 2018 - Healthy Lifestyle Lowers Odds of Breast Cancer’s Return
December 11, 2018 - New research identifies two genes linked to serious congenital heart condition
December 11, 2018 - NIH Director talks science, STEM careers with preteens
December 11, 2018 - Disabling a Cellular Antivirus System Could Improve Gene Therapy
December 11, 2018 - New tool swiftly provides accurate measure of patients’ cognitive difficulties
December 11, 2018 - NICE releases new guidelines for diagnosis and management of COPD
December 11, 2018 - Without Obamacare penalty, think it’ll be nice to drop your plan? Better think twice
December 11, 2018 - Researchers capture high-resolution X-ray and NMR image of key immune regulator
December 11, 2018 - Natural flavonoid is effective at treating leishmanisis infections, study shows
December 11, 2018 - Avoidant grievers unconsciously monitor and block mind-wandering contents, study shows
December 11, 2018 - Study identifies how hantaviruses infect lung cells
Logos Biosystems releases new electrophoretic tissue clearing system with twice the features in half the space

Logos Biosystems releases new electrophoretic tissue clearing system with twice the features in half the space

image_pdfDownload PDFimage_print

Logos Biosystems has released the latest system for electrophoretic tissue clearing, the X-CLARITY™ Tissue Clearing System II. The new system has been upgraded with new features that make it streamlined, easier to use and more intuitive.

The X-CLARITY II is a compact, all-in-one, solution for convenient, rapid and electrophoretic tissue clearing (ETC). The system provides excellent transparency and is compatible with multiple tissue types and sizes, including hard tissues such as bone. Enabling preparation of whole tissues for analysis, the X-Clarity II saves users having to waste valuable time on the preparation, sectioning, labeling and mounting of hundreds of ultra-thin tissue slices.

A new ETC tower has replaced the previous ETC controller and pump and the system is neatly contained, with the ETC chamber and buffer reservoir sitting in a custom tray.

Benchtop space is always at a premium in the lab, since more space means more equipment can be made available for users to expand their research. With this in mind, the sleek X-CLARITY II has been designed to halve the system’s footprint.

The X-CLARITY II boasts increased functionality, featuring both active and passive tissue clearing modes rather than just the one active mode.  Researchers can choose from multiple options to tailor the clearing mode to their sample.

In the passive clearing mode, users can define up to three different cycles, where time, pump speed and clearing temperatures can all be precisely set. In the active mode, the X-CLARITY II can be set to apply a constant current or voltage.

The introduction of an easy-to-use, interactive touchscreen interface to replace the buttons that were previously used, means operators can now control the entire system and all its settings in an intuitive and straightforward manner.

Graphic onscreen readouts of the clearing conditions can be displayed in real time, including, temperature, voltage and current. These can also be exported to maintain quality control and keep a record of the clearing procedures and parameters that have previously been run.

Since the ETC process generates a lot of heat, Logos Biosystems has integrated a buffer temperature control module in the control tower to replace the small cooling system that was previously in the chamber. Buffer circulates in the tower, heating or cooling the solution as required, before it passes through to the chamber.

The new temperature control system allows much faster and more precise temperature setting, providing researchers with peace of mind. The extra equipment that may previously have been needed to ensure samples are kept at the right temperature is no longer required.

X-CLARITY II Tissue Clearing Applications

Tissue clearing provides users with a way of producing high-fidelity 3D images of their samples, without damaging or altering the underlying structure of the organic matter.

The X-CLARITY Tissue Clearing System is currently being used for the DataBrain project at the Centro E Piaggio in Pisa, Italy to develop a high-fidelity map of the brain using data of the neurons in their native 3D arrangement.

It is also being used in support of regenerative spinal cord research at the École polytechnique fédérale de Lausanne (EPFL) in Lausanne, Switzerland, where the X-CLARITY clearing technique was used to model severe spinal cord injuries in rodents. A severe contusion abolished all motor cortex projections below injury, which led to paralysis.

However, electrochemical neuromodulation therapy reinforced these projections and the motor cortex immediately regained adaptive control over the paralyzed legs. Reorganization of the circuit mediated a motor cortex-dependent recovery of walking and swimming without neuromodulation being required.

At INSERM (National Institute of Health and Medical Research) in Lyon, France, the X-CLARITY has been used to investigate the molecular mechanisms that control cell death during ischemia-reperfusion.

Logos Biosystems

Logos Biosystems

Logos Biosystems is dedicated to the development and commercialization of innovative technologies to support the life science research community. Since 2008, Logos Biosystems has been developing a series of automated systems and imaging instruments for laboratories engaging in research with a cellular and molecular emphasis.

Tagged with:

About author

Related Articles