Dolomite Bio, a leading provider of high throughput single-cell technology today announced the release of its new Drop-seq datasets for single cell RNA-Seq. The data, generated on Dolomite Bio’s Nadia Instrument, demonstrates excellent single-cell RNA-seq performance with high gene capture, low doublet rates and cost-effective profiling.
The Nadia Instrument, a high-throughput platform for automated single-cell analysis, encapsulates up to 8 samples, in parallel, in under 20 mins. High quality single-cell data produced from the Nadia Instrument enables the analysis of thousands of single cells allowing scientists to identify and monitor cellular expression patterns. The data-sets generated on the Nadia Instrument showed exceptional quality and high reproducibility. Highlights include:
- Highly monodisperse droplets resulting in an ultra-low doublet rate of 6-7%
- Above market average gene capture, even at low sequencing depth
- Low duplication rate of PCR amplified transcripts resulting in cost-effective profiling
The high-quality single cell data has been generated using an improved workflow for generation of scRNA-seq libraries using the Dolomite Bio Nadia Instrument and the Drop-seq protocol (Macosko E., et al. “Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.” Cell 161:1202). The high-quality single cell DNA libraries were obtained by analyzing human and mouse mixed species sample on the Nadia Instrument.
In conclusion, the single-cell RNA-Seq application using the Drop-seq protocol has demonstrated very high-quality results using the Nadia Instrument. Data has not only shown to be extremely reproducible and cost-effective but also exceeding average market quality compared to similar products. The full analysis can be found on the Single cell RNA-Seq data sheet.