Breaking News
December 17, 2018 - Will saliva translate to a real diagnostic tool?
December 17, 2018 - DFG establishes nine new Research Units and one new Clinical Research Unit
December 17, 2018 - Assisted living’s breakneck growth leaves patient safety behind
December 17, 2018 - America’s teens report dramatic increase in their use of vaping devices in just one year
December 17, 2018 - Enlarged heart linked to a higher risk of dementia
December 17, 2018 - Prostate cancer detection using MRI now first-line investigation tool
December 17, 2018 - Adolescents who use cognitive reappraisal had better metabolic measures, shows study
December 17, 2018 - Probiotics may offer therapeutic benefits for biopolar patients
December 17, 2018 - Stealth BioTherapeutics Granted Fast Track Designation for Elamipretide for the Treatment of Dry Age-Related Macular Degeneration with Geographic Atrophy
December 17, 2018 - Studies reveal role of red meat in gut bacteria, heart disease development
December 17, 2018 - Eisai enters into agreement with Eurofarma for its anti-obesity agent lorcaserin
December 17, 2018 - Researchers use brain connectome to reassess neuroimaging findings of Alzheimer’s disease
December 17, 2018 - “Miracle” baby survives Ebola in Congo and rapid a new Ebola detection device
December 17, 2018 - Mechanisms behind neonatal diabetes uncovered
December 17, 2018 - AHF urges the WHO to expedite approval process for vaccine effective against Ebola
December 17, 2018 - Study finds misuse of benzodiazepines to be highest among young adults
December 17, 2018 - TGen receives PayPal grant to underwrite costs of genetic tests for children with rare disorders
December 17, 2018 - New research highlights why HIV-infected patients suffer higher rates of cancer
December 17, 2018 - Antibiotic-resistant bacteria could soon be targeted with Alzheimer’s drug
December 17, 2018 - Rutgers scientists take an important step in making diseased hearts heal themselves
December 17, 2018 - Tailored Feedback at CRC Screen Improves Lifestyle Behaviors
December 17, 2018 - Loss of two genes drives a deadly form of colorectal cancer, reveals a potential treatment
December 17, 2018 - How the Mediterranean Diet Can Help Women’s Hearts
December 17, 2018 - Sustained connections associated with symptoms of autism
December 17, 2018 - Concussion rates among young football players were higher than previously reported
December 17, 2018 - Cresco Labs granted approval to operate marijuana dispensary in Ohio
December 17, 2018 - Study provides insight into health risks facing new mothers
December 17, 2018 - AMSBIO expands Wnt signaling pathway product range to aid research
December 16, 2018 - Surgical treatment unnecessary for many prostate cancer patients
December 16, 2018 - Excess weight responsible for cancers globally finds report
December 16, 2018 - Regular sex associated with greater enjoyment of life in seniors
December 16, 2018 - Social stigma contributes to poor mental health in the autistic community
December 16, 2018 - Multidisciplinary team successfully performs complex surgery on patient suffering from enlarged skull
December 16, 2018 - Experts analyze data that can guide antidepressant discontinuation
December 16, 2018 - Menlo Therapeutics’ Successful Phase 2 Clinical Trial of Serlopitant Demonstrates Reduction of Pruritus Associated with Psoriasis
December 16, 2018 - Siblings of children with autism or ADHD are at elevated risk for both disorders
December 16, 2018 - New project aims to understand why and how metabolic disorders develop in patients
December 16, 2018 - Diets containing GM maize have no harmful effects on health or metabolism of rats
December 16, 2018 - Are doctors and teachers confusing immaturity and attention deficit?
December 16, 2018 - Hearing loss linked with increased risk for premature death
December 16, 2018 - Chromatrap buffer reagents for lysing cells offer many benefits
December 16, 2018 - Young Breast Cancer Patients Face Higher Risk for Osteoporosis
December 16, 2018 - 3-D printing offers helping hand to people with arthritis
December 16, 2018 - Community Health Choice helps manage complex and chronic care conditions
December 16, 2018 - Regular trips out could dramatically reduce depression in older age
December 16, 2018 - CWRU to use VivaLNK’s Vital Scout device for stress study in student athletes
December 16, 2018 - ‘Easy Way Out’? Stigma May Keep Many From Weight Loss Surgery
December 16, 2018 - Gout drug may protect against chronic kidney disease
December 16, 2018 - Talking about memories enhances the wellbeing of older and younger people
December 16, 2018 - Occupational exposure to pesticides increases risk for cardiovascular disease among Latinos
December 16, 2018 - A biomarker in the brain’s circulation system may be Alzheimer’s earliest warning
December 16, 2018 - Magnesium may play important role in optimizing vitamin D levels, study shows
December 16, 2018 - The effect of probiotics on intestinal flora of premature babies
December 16, 2018 - Parents spend more time talking with kids about mechanics of using mobile devices
December 16, 2018 - Biohaven Announces Positive Results from Ongoing Rimegepant Long-Term Safety Study
December 16, 2018 - Arterial stiffness may predict dementia risk
December 16, 2018 - Study explores link between work stress and increased cancer risk
December 16, 2018 - Sex work criminalization linked to incidences of violence finds study
December 16, 2018 - Johns Hopkins researchers discover swarming behavior in fish-dwelling parasite
December 16, 2018 - Schistosomiasis prevention and treatment could help control HIV
December 16, 2018 - Early postpartum opioids linked with persistent usage
December 16, 2018 - Johns Hopkins researchers identify molecular causes of necrotizing enterocolitis in preemies
December 16, 2018 - Advanced illumination expands capabilities of light-sheet microscopy
December 16, 2018 - Alzheimer’s could possibly be spread via contaminated neurosurgery
December 16, 2018 - Unraveling the complexity of cancer biology can prompt new avenues for drug development
December 16, 2018 - Inflammatory Bowel Disease, Prostate Cancer Linked
December 16, 2018 - Cannabis youth prevention strategy should target mental wellbeing
December 15, 2018 - Recent developments and challenges in hMAT inhibitors
December 15, 2018 - Sewage bacteria found lurking in Hudson River sediments
December 15, 2018 - CDC selects UMass Amherst biostatistician model that helps predict influenza outbreaks
December 15, 2018 - Researchers reveal brain mechanism that drives itch-evoked scratching behavior
December 15, 2018 - New computer model helps predict course of the disease in prostate cancer patients
December 15, 2018 - Obesity to Blame for Almost 1 in 25 Cancers Worldwide
December 15, 2018 - How the brain tells you to scratch that itch
December 15, 2018 - New findings could help develop new immunotherapies against cancer
December 15, 2018 - World’s largest AI-powered medical research network launched by OWKIN
December 15, 2018 - Young people suffering chronic pain battle isolation and stigma as they struggle to forge their identities
December 15, 2018 - Lifespan extension at low temperatures depends on individual’s genes, study shows
December 15, 2018 - New ingestible capsule can be controlled using Bluetooth wireless technology
December 15, 2018 - Researchers uncover microRNAs involved in the control of social behavior
Researchers delineate architecture of nuclear pore complex in yeast cells

Researchers delineate architecture of nuclear pore complex in yeast cells

image_pdfDownload PDFimage_print

Like an island nation, the nucleus of a cell has a transportation problem. Evolution has enclosed it with a double membrane, the nuclear envelope, which protects DNA but also cuts it off from the rest of the cell. Nature’s solution is a massive–by molecular standards–cylindrical configuration known as the nuclear pore complex, through which imports and exports travel, connecting the bulk of the cell with its headquarters.

In research described March 14 in Nature, scientists at Rockefeller University and their colleagues have delineated the architecture of the nuclear pore complex in yeast cells. The biological blueprint they uncovered shares principles sometimes seen on a much larger scale in concrete, steel, and wire.

“It reminds us of a suspension bridge, in which a combination of sturdy and flexible parts produce a stress-resilient structure,” says Michael P. Rout, who led the work together with Brian T. Chait.

The pore complex contains 552 component proteins, called nucleoporins, and scientists hadn’t previously known how they all fit together. It took a combination of approaches to assemble a comprehensive map of these pieces. The researchers hope this new molecular structure will enable new studies of how the nuclear portal functions normally, and how defects in it lead to diseases such as cancer.

A milestone

The pore complex first emerged when single-celled organisms–the only living things at the time–acquired special compartments containing organ-like structures, including the nucleus, which houses the cell’s genetic code.

It serves not only as a conduit to and from the nucleus, but also as a checkpoint regulating what passes in and out. Genetic instructions transcribed into RNA are allowed to exit, for example, while proteins needed inside the nucleus may enter. Other things, such as viruses bent on taking over the cell, are kept at bay.

Rout and Chait began mapping this ancient structure more than 20 years ago, knowing the project could well span decades since the target of their curiosity is not easily defined.

More than a third of the pore complex can move about, and this flexibility, along with the structure’s immense size and the constant stream of traffic passing through it, meant that no single approach to mapping it would work. “In the end, we used everything we could lay our hands on, brought the results together, and integrated them into a single structure,” says Chait, who is Rockefeller’s Camille and Henry Dreyfus Professor.

Together with researchers at the University of California, San Francisco; Boston University Medical School; and Baylor College of Medicine, the team was able to determine the type and amount of each nucleoporin and their proximities to one another, as well as the weight and shape of the whole complex.

This data allowed them to visualize the anatomy of many of the individual pore components and to place them all within the pore complex. They uncovered a complicated ringed structure containing rigid, diagonal columns and flexible connectors that evoke the towers and cables of human-made structures like the Golden Gate Bridge.

The resulting map is a breakthrough in a line of investigation with a deep Rockefeller history. The pore complex first came into human view in the 1950s, when a university scientist, Michael Watson, observed small densities dotting the surface of the nuclear envelope. And about two decades later, the lab of Günter Blobel, who passed away last month, was among the first to discover individual nups and then determine their structure.

A new starting point

When it comes to the pore complex, yeast has a considerable amount in common with us. When the team compared their data with structural findings from human pore complexes, they found similar elements arranged somewhat differently. The resemblance suggests the yeast pore complex could be useful for research relevant to humans.

And there’s a lot of such research to be done. Defects in the pore complex and its components have been linked to a host of diseases, including autoimmune disorders and cancer; meanwhile, viruses have evolved ways to sneak past it altogether. But the details of these malfunctions and blind spots are often obscure.

The new yeast structure may help. With it, the team found they could map sites that are altered in some cancers–evidence, they say, that the yeast pore complex can be used to test how factors like stress, drugs, or mutations change the human structure, and so aiding efforts to understand and treat disease.

Source:

https://www.rockefeller.edu/news/21521-scientists-map-portal-cells-nucleus/

Tagged with:

About author

Related Articles