Breaking News
July 23, 2018 - Yeast species used in biotechnology, food industries causes drug-resistant yeast infections
July 23, 2018 - Breath analyses may help detect pancreatic cancer earlier, study shows
July 23, 2018 - Study reports data on dementia prevalence in sexual minority older adults
July 23, 2018 - Children of mothers with type 1 diabetes at greater risk of being overweight
July 23, 2018 - Deep brain stimulation found to improve diabetes symptoms
July 23, 2018 - New breeding technologies could enhance shape, size, color, and health benefits of produce
July 23, 2018 - Pfizer announces FDA approval of biosimilar to Neupogen
July 23, 2018 - Key questions to consider for sustainable management of environmental risks in Europe
July 23, 2018 - Vaxart Announces Publication of the Phase 1 Results of its Oral Norovirus Tablet Vaccine in Journal of Clinical Investigation Insight
July 23, 2018 - Joslin researchers report excessive fetal growth despite well-controlled type 1 diabetes
July 23, 2018 - Former inmates more likely to die of opioid overdose after prison release
July 23, 2018 - New study offers robust insights into under-treatment of women with heart attacks
July 23, 2018 - Doctors’ ‘gut feelings’ play key role in medical decision making, finds study
July 23, 2018 - Tooth enamel that regrows? Researcher says revolutionary gel could make it possible
July 23, 2018 - ECU researchers develop world’s first blood test that detects melanoma in its early stages
July 23, 2018 - Researchers explore future of houseplants as functional sirens of home health
July 23, 2018 - No Outcome Differences Based on Anesthesia Team Make-Up
July 23, 2018 - Getting to the heart of congenital cardiac defects
July 23, 2018 - Healthy behaviors are not effective in preventing gestational diabetes in obese women
July 23, 2018 - Top of Teachers’ To-Do List: Focus on the Positives
July 23, 2018 - LDL quality is a novel, modifiable cardiovascular risk marker
July 22, 2018 - Researchers identify enzyme as potential new drug target for blood disorders
July 22, 2018 - Research shows that neurons can encode more than one signal at a time
July 22, 2018 - Exposure to secondhand smoke increases the risk of stillbirth
July 22, 2018 - Skin conditions by the numbers
July 22, 2018 - Brain tumour chemotherapy now available to even more NHS patients
July 22, 2018 - Researchers find mugwort pollen as major source of airborne endotoxins
July 22, 2018 - Occupational safety and health at workplace
July 22, 2018 - Pfizer And Lilly Announce Positive Top-Line Results From Phase 3 Trial Of Tanezumab For The Treatment Of Osteoarthritis (OA) Pain
July 22, 2018 - Early supper associated with lower risk of breast and prostate cancer
July 22, 2018 - Survey results identify major inequalities in acute stroke treatment across Europe
July 22, 2018 - Researchers discover promising treatment for genetic form of autism spectrum disorder
July 22, 2018 - Prenatal Depression More Common in Young Moms Today
July 22, 2018 - What helps adults with autism get and keep a job?
July 22, 2018 - Pediatric NEXUS Head CT DI guides selective imaging decisions in blunt trauma patients
July 22, 2018 - Novel tool predicts genes that cause disease due to production of truncated proteins
July 22, 2018 - AHA: Vaping Tied to Blood Clots — in Mice
July 22, 2018 - Study finds therapy dogs effective in reducing symptoms of ADHD
July 22, 2018 - Scientists find reason why malarial parasites are faster than immune cells
July 22, 2018 - Researchers gain more insight into how FUS protein causes ALS and FTLD
July 22, 2018 - Frequency of joint replacements halved in rheumatoid arthritis patients between 1997-2010
July 22, 2018 - QUT researcher highlights growing impact of non-prescription antibiotics supply in pharmacies
July 22, 2018 - UK health communication researcher seeks solutions for disposing leftover medicine
July 22, 2018 - Pfizer Initiates Pivotal Phase 3 Program for Investigational Hemophilia B Gene Therapy Fidanacogene Elaparvovec
July 22, 2018 - Mutation discovered to protect against Alzheimer’s disease in mice
July 22, 2018 - Researchers reveal how patients in urban areas develop multiple, long-term conditions
July 22, 2018 - Replacing conventional cancer treatment with complementary therapy linked to increased risk of death
July 22, 2018 - Study uncovers molecular key for delaying progression of multiple sclerosis
July 22, 2018 - Availability of athletic trainer in high school reduces injury rates in girls’ sports, shows study
July 22, 2018 - FDA Approves Krintafel (tafenoquine) for the Radical Cure of Plasmodium vivax Malaria
July 22, 2018 - Novel nuclear medicine probe will help assess new drugs for neurodegenerative diseases
July 22, 2018 - Physical activity even during exposure to air pollution can reduce risk of heart attack
July 22, 2018 - Scientists discover protein regulator of myelin production
July 22, 2018 - Sleep disturbances associated with higher dementia risk
July 22, 2018 - Scientists move one step further in developing eye drops to treat age-related macular degeneration
July 22, 2018 - Five-Year Stroke Rates Lower After PCI Versus CABG
July 21, 2018 - Alopecia areata – Genetics Home Reference
July 21, 2018 - Study identifies overdose risk factors in youth with substance use disorders
July 21, 2018 - Drug in clinical trials for Parkinson’s disease offers hope for treating heart failure
July 21, 2018 - Coupling free malaria tests with diagnosis-dependent vouchers can improve rational use of ACTs
July 21, 2018 - Sweetness depends on molecular interactions between specific sugars and water in saliva
July 21, 2018 - Muscle fitness is strongly associated with improved rate of ageing in the brain
July 21, 2018 - Resetting E-Prescriptions for Opioids Helps Curb Use: Study
July 21, 2018 - Overuse of antibiotics not what the doctor ordered
July 21, 2018 - Bundled-payment system did not lower costs for serious medical conditions, shows study
July 21, 2018 - Therapy dogs found to be effective in reducing ADHD symptoms in children
July 21, 2018 - Could rotating multiple therapists better treat PTSD patients?
July 21, 2018 - Binge drinking impairs working memory in adolescent brain
July 21, 2018 - Dying at home could be beneficial for terminally ill cancer patients and their relatives
July 21, 2018 - Researchers identify subtypes of retinal ganglion cells using single-cell RNA sequencing
July 21, 2018 - Study uncovers opportunities to reduce death by suicide among cancer patients
July 21, 2018 - Genetic sequencing reveals new clues to aggressiveness of prostate cancer
July 21, 2018 - BioSight Launches a Phase 2b Clinical Trial of BST-236 as a First-Line Treatment of Acute Myeloid Leukemia
July 21, 2018 - First major study comparing robotic to open surgery published in The Lancet
July 21, 2018 - ADHD medications may fail to improve cognition in healthy college students, study shows
July 21, 2018 - Intervention program that includes a personalized app could benefit teens with suicidal thoughts
July 21, 2018 - Researchers identify new compound that protects against neurodegeneration
July 21, 2018 - Gene therapy may hold potential to treat people with spinal cord injuries
July 21, 2018 - FDA Approves Nivestym (filgrastim-aafi), a Biosimilar to Neupogen
July 21, 2018 - Surgeons have substantial impact on genetic testing in breast cancer patients who need it
Live-cell imaging capabilities of the InVi SPIM microscope announced by Luxendo

Live-cell imaging capabilities of the InVi SPIM microscope announced by Luxendo

image_pdfDownload PDFimage_print

Luxendo, a Bruker company, today announced the extension of the InVi SPIM microscope to include full incubation capabilities, as well as flexible illumination and detection optics. Luxendo’s proprietary single-plane illumination microscopy (SPIM) technique significantly reduces sampling times over conventional laser scanning confocal microscopes, while reducing phototoxicity and damaging side effects on living specimens.

The new technology also enables the fastest scan speeds for volumetric imaging of small organisms, cell monolayers, and cleared tissue. InVi SPIM combines these capabilities with perfect environmental conditions and gentle sample handling to create an optimized platform for ‘pure life imaging’.

We have been using the InVi SPIM light-sheet microscope to image breast tumor organoids derived from primary mammary epithelial cells” said Dr. Martin Jechlinger, Group Leader for the Cell Biology and Biophysics Unit, EMBL Heidelberg, Germany, and EMBL Rome.

Ease of mounting the sample has allowed us to acquire fantastic images of tumor progression and regression on the InVi. Now, with its full incubation functionality and its improved illumination and detection concept, we can run long-term experiments on tumorigenesis that are not feasible on any other microscope system.”

“In live-cell imaging, the effect of photons to the specimen can have a big impact on the cells’ viability and unabridged development,” said Dr. Andreas Pfuhl, Managing Director of Luxendo. “With the inverted InVi SPIM platform combining precise environmental control with highly flexible optical settings, we can ensure that the observation method is not interfering with the cells’ physiological behavior.”

The InVi SPIM’s successful handling of extremely sensitive specimens, such as embryonic cells during their first divisions, has been proven in recent publications (Strnad et al., Nature Methods 2015; Reichmann et al., Science 2018). Other samples, such as tumor spheroids, organoids, and all kinds of culture cell lines, can now be imaged with more than 80 frames per second in full frame 2048 x 2048-pixel resolution up to 500 frames per second in 192 x 2048 resolution.

About InVi SPIM

The InVi SPIM microscope enables fast 3D imaging with extremely gentle sample handling for cell cultures and developing embryonic samples. Easy access to the sample chamber, maximized photo-efficiency, and short illumination times enable long-term imaging without harming live specimens. Simultaneous dual-channel detection allows the observation of multiple fluorophores without compromising acquisition speed. At the same time, the light-sheet length and thickness can be adapted to the two available magnification steps. While the specimen is kept in a specific sample carrier that serves as a “curved coverslip,” the environmental control is thoroughly monitored and adjusted for humidity, temperature, carbon dioxide and oxygen concentration. This stabilizes the experimental conditions in cellular imaging in such a way that observation times for up to 72 hours can be achieved.

Tagged with:

About author

Related Articles