Breaking News
March 23, 2019 - Excess hormones could cause a condition that can lead to blindness in women, study finds
March 23, 2019 - Dramatic shifts in first-time opioid prescriptions bring hope, concern
March 23, 2019 - Antidepressant drugs may not work when neurons are out of shape
March 23, 2019 - TTUHSC El Paso to establish endowed chair in neurology through a major grant
March 23, 2019 - New device approved by FDA for treating patients with moderate-to-severe heart failure
March 23, 2019 - People with peripheral artery disease have lower Omega-3 Index, shows research
March 23, 2019 - Trigger warnings have minimal impact on how people respond to content, shows research
March 23, 2019 - Gilead Announces Data From Two Studies Supporting Further Development of GS-6207, a Novel, Investigational HIV-1 Capsid Inhibitor as a Component of Future Long-Acting HIV Therapies
March 23, 2019 - Selfish genetic elements amplify inflammation and age-related diseases
March 23, 2019 - Study provides new understanding of how the brain recovers from damage caused by stroke
March 23, 2019 - CRISPR/Cas libraries could revolutionize drug discovery
March 23, 2019 - Allergic reaction during pregnancy may alter sexual-development in offspring’s brain
March 23, 2019 - Seeing through a robot’s eyes helps those with profound motor impairments
March 23, 2019 - Recent research shows that ease of breastfeeding after C-section differs culturally
March 23, 2019 - Newly discovered parameters offer more control over efficient release of drugs
March 23, 2019 - ‘De-tabooing’ of abortion- Women would like more support from health care community
March 23, 2019 - Anti-TB drugs can increase susceptibility to Mtb reinfection
March 23, 2019 - New survey indicates need of attention to neglected tropical diseases
March 23, 2019 - Innovative in vitro method to develop easy-to-swallow medicine for children and older people
March 23, 2019 - Sugary drinks could raise risk of early deaths finds study
March 23, 2019 - Lian wins ENGINE grant for stem-cell-based therapy to treat Type 1 diabetes
March 23, 2019 - Overall, Physicians Are Happy and Enjoy Their Lives
March 23, 2019 - Researchers discover how blood vessels protect the brain during inflammation
March 23, 2019 - CDC study shows modest improvement in optimal hospital breastfeeding policy
March 23, 2019 - Family-based prevention program to reduce alcohol use among older teens
March 23, 2019 - Remote monitoring of implanted defibrillators in heart failure patients prevents hospitalizations
March 23, 2019 - Appropriate doffing of personal protective equipment may reduce healthcare worker contamination
March 23, 2019 - Window screens can suppress mosquito populations, reduce malaria in Tanzania
March 23, 2019 - Researchers discover new biomarker for postoperative liver dysfunction
March 23, 2019 - Pregnancy history may be linked to cognitive function in older women, finds study
March 23, 2019 - Study shows ticagrelor is equally safe and effective as clopidogrel after heart attack
March 23, 2019 - FDA Approves First Drug for Postpartum Depression, Zulresso (brexanolone)
March 23, 2019 - New guidelines outline new treatment management for psoriasis
March 23, 2019 - Thermally abused cooking oil may promote progression of breast cancer
March 23, 2019 - High-fructose corn syrup fuels growth of colon tumors in mice
March 23, 2019 - Partnership aims at establishing best practices to promote diversity in clinical trials
March 23, 2019 - New study examines presence of microbes in tap water from residences, office buildings
March 23, 2019 - Early life trauma may affect brain structure, contribute to major depressive disorder
March 23, 2019 - NIH starts clinical trial of drug to treat cravings associated with opioid use disorder
March 23, 2019 - Cervix bacteria, immune factors could be a warning signal of premature birth, reports new research
March 23, 2019 - Worst-ever emergency care performance figures underscore the need to focus on staffing
March 23, 2019 - The Current issue of “The view from here” is concerned with Cancer
March 23, 2019 - Mouse model validates how ‘good’ and ‘bad’ bacteria affect acne
March 23, 2019 - Individual amygdala neurons respond to touch, imagery and sounds
March 23, 2019 - Combination of two topical creams can prevent cancer
March 23, 2019 - Study suggests depression screening when assessing African-Americans for schizophrenia
March 23, 2019 - New electronic support system for choosing drug treatment based on patient’s genotype
March 23, 2019 - First-of-its-kind study provides pregnancy statistics of imprisoned U.S. women
March 23, 2019 - Marinus Pharmaceuticals Initiates Phase 3 Study in Children with PCDH19-Related Epilepsy
March 23, 2019 - Laparoscopy: MedlinePlus Lab Test Information
March 23, 2019 - Shellfish allergies: can they be treated?
March 23, 2019 - Toilet seat heart monitoring system
March 23, 2019 - Researchers identify way to improve common treatment for PTSD
March 23, 2019 - High potency cannabis use linked to psychosis finds study
March 23, 2019 - Evoke Pharma Submits Response to FDA Review Letter for Gimoti NDA
March 23, 2019 - Tracking HIV’s ever-evolving genome in effort to prioritize public health resources
March 23, 2019 - Scientists grow most sophisticated brain organoid to date
March 23, 2019 - ADHD drug raising risk of psychosis
March 22, 2019 - FDA approves brexanolone, first drug developed to treat postpartum depression
March 22, 2019 - Gruesome cat and dog experiments by the USDA exposed
March 22, 2019 - Ball pits used in children’s physical therapy may contribute to germ transmission
March 22, 2019 - Long-term use of inexpensive weight-loss drug may be safe and effective
March 22, 2019 - FDA Approves Sunosi (solriamfetol) for Excessive Daytime Sleepiness Associated with Narcolepsy or Obstructive Sleep Apnea
March 22, 2019 - Anti-Müllerian Hormone Test: MedlinePlus Lab Test Information
March 22, 2019 - Finding the right exercise, diet aids for HIV patients
March 22, 2019 - Health Plans For State Employees Use Medicare’s Hammer On Hospital Bills
March 22, 2019 - Researchers develop new tool for imaging large groups of neurons in living animals
March 22, 2019 - Certain bacteria and immune factors in vagina may cause or protect against preterm birth
March 22, 2019 - Research identifies guidelines for prioritizing hepatitis C treatment in U.S. prisons
March 22, 2019 - Novel breath test could pave new way to non-invasively measure gut health
March 22, 2019 - Pharmaceutical and personal care products may result in new contaminants in waterways
March 22, 2019 - New model could revolutionize the way researchers investigate spread of pathogens
March 22, 2019 - MSU professor receives NSF CAREER grant for biosensor diagnostics
March 22, 2019 - High-fat, high-sugar diet in mouse mothers causes problems in the hearts of offspring
March 22, 2019 - ACC: Catheter Ablation Does Not Cut Mortality, Stroke in A-Fib
March 22, 2019 - Wiedemann-Rautenstrauch syndrome – Genetics Home Reference
March 22, 2019 - Health insurance is not assurance of healthcare
March 22, 2019 - Supporting “curiosity-driven research” at the Discovery Innovation Awards
March 22, 2019 - Must-Reads Of The Week (Some Flying Below The Radar)
March 22, 2019 - Newly engineered nanoscale protein micelles can be tracked by MRI
An Insider’s Guide to the chemagic™ Prime™ Junior Instrument

An Insider’s Guide to the chemagic™ Prime™ Junior Instrument

image_pdfDownload PDFimage_print

In this interview, Masoud Toloue, vice president of Applied Genomics at PerkinElmer, provides an overview of the chemagic™ Prime™ Junior Instrument and gives his inside tips for scientists thinking of purchasing this product.

How are nucleic acids typically used by researchers?

Recent advances in molecular biology are making a significant impact on clinical diagnosis, drug discovery and other related areas. Methods used by researchers in these fields include NGS for transcriptomics, metagenomics analysis, long-read sequencing, as well as targeted sequencing and PCR analysis.

Nucleic acidsvchal | Shutterstock

What are the main challenges for researchers when carrying out an assay such as PCR?

Most applications used for analyzing DNA or RNA require high quality input, and many of the challenges researchers face when analyzing nucleic acids are generated during the isolation step. These issues include inhibitor carryover, the fragmentation of the nucleic acid during isolation, cross contamination, and processing bottlenecks.

What is the chemagic Prime Junior instrument and how does it address these issues?

PerkinElmer’s chemagic Prime Junior instrument, in combination with the chemagic kits, enables a streamlined workflow from primary sample to ready to use nucleic acids. The instrument performs eluate handling and has reserved deck space for automated PCR, NGS or general assay setup. It is compatible with any human sample materials and delivers clean, high molecular weight DNA ready to use for PCR and NGS analysis.

The chemagic Prime Junior instrument uses proprietary chemagen™ technology to capturing nucleic acids using M-PVA magnetic beads, which are subsequently attracted to metal rods transiently magnetized by an electromagnet. The magnetized rods transfer the M-PVA magnetic beads through the different processing solutions, avoiding potential sample cross-contamination by eliminating the liquid transfer steps used with other nucleic acid extraction technologies.

The rod rotation is switched on after deactivation of the electromagnet, leading to efficient and homogeneous resuspension of the particles during the sample resuspension and the subsequent washing steps. This proprietary process allows the chemagic Prime Junior instrument to deliver high yields of pure nucleic acids, which are not fragmented during processing. Automating nucleic acid isolation with the instrument minimizes user intervention and extraction-to extraction variability.

Which applications are currently available for the chemagic Prime Junior Instrument?

To serve the unique needs of users and keep the operation as convenient as possible, PerkinElmer offers a choice between two kits, covering a wide range of human sample materials, independent of volumes and targets. This reduces costs and facilitates the warehousing of kits, available for the isolation of genomic DNA or viral and bacterial DNA and RNA.

How easy is it to set up an assay using this instrument?

The set-up for 48 samples can be completed in less than 10 minutes. The operator places the primary samples, consumables and magnetic beads on the deck of the chemagic Prime Junior instrument. Next, the kit reagents are dispensed automatically according to the number of samples used in the run. PerkinElmer’s easy-to-use software also guides the operator through each of these steps.

If you could send one message to a scientist who is thinking of purchasing this instrument, what would it be?

The chemagic Prime Junior Instrument was designed to alleviate many of the challenges researchers face by reducing inhibitor carryover, fragmentation of the nucleic acid during isolation, cross-contamination and processing bottlenecks.

About Masoud Toloue

Photo of Dr. Masoud ToloueMasoud Toloue, Ph.D., is the vice president and general manager of Applied Genomics at PerkinElmer. Masoud graduated from the University of Buffalo with a Ph.D. in Molecular Biology. He completed a Postdoctoral Fellowship in Biochemistry at the University of Texas Health Science Center, San Antonio, where he studied membrane protein structure and function. He is the recipient of several grants and patents from the National Institutes of Health and the National Science Foundation.

Tagged with:

About author

Related Articles