Breaking News
February 23, 2019 - Intensive therapy during early stages of MS leads to better long-term outcomes
February 23, 2019 - Prenatal Fluconazole Exposure Increases Neonatal Risks
February 23, 2019 - Mental Health Screening: MedlinePlus Lab Test Information
February 23, 2019 - Study suggests birth mechanics are part of the process that leads to autism
February 23, 2019 - Unhealthy diet linked to poor mental health
February 23, 2019 - Study gives a snapshot of crocodile evolution
February 23, 2019 - Research finds steep rise in self-poisonings among young people
February 23, 2019 - American Gastroenterological Association announces “AGA Future Leaders Program”
February 23, 2019 - Scientists uncover new mechanisms regulating neural stem cells
February 23, 2019 - Combinations of certain insecticides turn out to be lethal for honeybees
February 23, 2019 - AHA News: Why Are Black Women at Higher Risk of Dying From Pregnancy Complications?
February 23, 2019 - NIMH » Anxiety Disorders
February 23, 2019 - Autistic people urgently need access to tailored mental health support
February 23, 2019 - Newly designed molecule could benefit people with Friedrich’s Ataxia
February 23, 2019 - Chinese CRISPR twins may have better cognition and memory
February 23, 2019 - Study finds new genetic clues associated with asthma in African ancestry populations
February 23, 2019 - Fetal signaling pathways may offer future opportunities to treat lung damage
February 23, 2019 - Early-stage osteoarthritis drug wins prestigious innovation award
February 23, 2019 - Researchers report positive findings with dasotraline for ADHD in children ages 6-12
February 23, 2019 - News study reanalyzes the effects of noncaloric sweeteners on gut microbiota
February 23, 2019 - New device allows scientists to reproduce blow effects on the heart in lab
February 23, 2019 - Holy herb identified as a potential treatment for Alzheimer’s disease
February 23, 2019 - New technology platform digitally counts growth factors in single cells
February 23, 2019 - Surgery and other treatments offer viable options for adult scoliosis
February 23, 2019 - Reduced antibody adaptability may make the elderly more vulnerable to influenza
February 23, 2019 - Neighborhood income, education associated with risk of disability progression in MS patients
February 23, 2019 - Endocrine Society opposes new rule that restricts access to Title X Family Planning Program
February 23, 2019 - 2019 guidelines for management of patients with atrial fibrillation
February 23, 2019 - Surprise rheumatoid arthritis discovery points to new treatment for joint inflammation
February 23, 2019 - A just-right fix for a tiny heart
February 23, 2019 - UMass Amherst scientist explores role of citrus peel in decreasing gut inflammation
February 23, 2019 - Owlstone Medical and Shanghai Renji Hospital collaborate to initiate breath biopsy lung cancer trial
February 23, 2019 - AMSBIO’s comprehensive portfolio of knock-out cell lines and lysates
February 23, 2019 - New app reliably determines physicians’ skills in forming accurate, efficient diagnoses
February 23, 2019 - Peripheral nerve injury can trigger the onset and spread of ALS, shows study
February 23, 2019 - Researchers uncover mechanisms that prevent tooth replacement in mice
February 23, 2019 - Once-a-day capsule offers new way to reduce symptoms of chronic breathlessness
February 23, 2019 - FDA Adds Boxed Warning for Increased Risk of Death with Gout Medicine Uloric (febuxostat)
February 23, 2019 - Phone-based intervention aids rheumatoid arthritis care
February 23, 2019 - Opioid epidemic makes eastern inroads and targets African-Americans
February 23, 2019 - New identified biomarker predicts patients who might benefit from HER2-targeted agents
February 23, 2019 - Study offers new insights into mechanisms of changes in erythrocytes under stress
February 23, 2019 - Antipsychotic polypharmacy may be beneficial for schizophrenia patients
February 23, 2019 - Researchers investigate how marijuana and tobacco co-use affects quit attempts by smokers
February 23, 2019 - Patients with diabetes mellitus have high risk of stable ischemic heart disease
February 23, 2019 - Transparency on healthcare prices played key role in Arizona health system’s turnaround
February 23, 2019 - A comprehensive, multinational review of peppers around the world
February 23, 2019 - Study finds modest decrease in burnout among physicians
February 23, 2019 - A simple change can drastically reduce unnecessary tests for urinary tract infections
February 23, 2019 - Deep Learning-Enhanced Device Detects Diabetic Retinopathy
February 23, 2019 - Researchers discover new binding partner for amyloid precursor protein
February 23, 2019 - Modest decrease seen in burnout among physicians, researchers say | News Center
February 23, 2019 - Transplanting bone marrow of young mice into old mice prevents cognitive decline
February 23, 2019 - Mogrify to accelerate novel IP and cell therapies using $3.7m USD funding
February 23, 2019 - Johns Hopkins study describes cells that may help speed bone repair
February 23, 2019 - Scientists demonstrate influence of food odors on proteostasis
February 23, 2019 - Researchers unlock the secret behind reproduction of fish called ‘Mary’
February 23, 2019 - Acupuncture Could Help Ease Menopausal Symptoms
February 23, 2019 - Researchers use AI to detect early signs of Alzheimer’s
February 23, 2019 - On recovery, vulnerability and ritual: An exhibit in white | News Center
February 23, 2019 - Memory Stored in Unexpected Region of the Brain
February 23, 2019 - Several health experts worldwide gather at EUDONORGAN event
February 23, 2019 - Discovery of potent compound in native California shrub may lead to treatment for Alzheimer’s
February 22, 2019 - Researchers create new map of the brain’s own immune system
February 22, 2019 - ICHE’s reviews on surgical infections, unnecessary urine tests, and nurses’ role in antibiotic stewardship
February 22, 2019 - UK Research and Innovation invests £200 million to create new generation of AI leaders
February 22, 2019 - Takeda collaboration to boost fight against Alzheimer’s and other neurodegenerative diseases
February 22, 2019 - Heavy drinking may change DNA, leading to increased craving for alcohol
February 22, 2019 - U.S. opioid deaths jump fourfold in 20 years; epidemic shifts to Eastern states | News Center
February 22, 2019 - 5 Questions with William Turner on Diversity in Medicine
February 22, 2019 - HHS Finalizes Rule Seeking To Expel Planned Parenthood From Family Planning Program
February 22, 2019 - Researchers uncover biochemical pathway that may help identify drugs to treat Alzheimer’s
February 22, 2019 - Biologist uses new grant to find ways to eliminate schistosomiasis
February 22, 2019 - Bag-mask ventilation to help patients breathe during intubation prevents complications
February 22, 2019 - AbbVie Announces New Drug Application Accepted for Priority Review by FDA for Upadacitinib for Treatment of Moderate to Severe Rheumatoid Arthritis
February 22, 2019 - Nature versus nurture and addiction
February 22, 2019 - New website connects researchers with data experts, resources | News Center
February 22, 2019 - Today’s Concerns About Drug Prices Echo The Past
February 22, 2019 - CT and Doppler equipment have low accuracy in detecting cerebral vasospasm and ischemia
February 22, 2019 - Study finds out similarity in function between healthy retina cell and tumor cell
Ural Federal University scientists synthesize a group of multi-purpose fluorophores

Ural Federal University scientists synthesize a group of multi-purpose fluorophores

image_pdfDownload PDFimage_print

A group of scientists from Russia’s Ural Federal University, headed by Professor Grigory Zyryanov, synthesized a group of multi-purpose fluorophores. The scientists told about the content and results of their research on the unique technology in a respected international scientific journal Dyes and Pigments.

‘Fluorophores are powders of different colors, which emit visible radiation (in other words – glow) when exposed to optical emission of a certain band. This phenomenon is called “cold light” or fluorescence,’ Zyryanov explains.

Fluorophores are called dyes because they are capable of staining biological objects: for instance, cells, including those affected by pathological processes. It is vital that the dyes, firstly, have the property of selectivity, meaning they stain, say, either healthy or diseased cells only. Secondly, they should be bright, high-contrast, and suitable for observation, since some biological tissues are naturally fluorescent.

The fluorophore is placed in a solvent, preferably water, then either the tested biological object is immersed in the resulting solution, or the solution is applied to the object. In this case, certain electrostatic, donor-acceptor interactions occur between the fluorophore and the bioobject: the fluorophore molecules are captured by receptor fragments of biomolecules (the scientific term for the substance being captured is ligand). As a result, supramolecular structures, (complexes based on non-covalent (weak, reversible) bonds) are formed.

By the way, many of the vital human body functions are controlled mainly by this kind of non-covalent interactions, and we are in some sense unsteady “construction kits”.

‘Depending on the type and intensity of the “communication” with the processed biological object, either the shift in the fluorescence band (in other words, the change in fluorophore’s color, for example, from blue to green or red), or the fluorescent enhancement/quenching occurs. Scientists call this dynamics a change in the physical signal emanating from a fluorophore molecule due to the interaction with the biological object under observation,’ Ural Federal University professor explains.

Such reaction indicates, for example, the area affected by pathogenic, particularly cancerous, cells (or, vice versa, healthy tissue surrounded by pathology is brightly stained). Thus, it is possible not only to establish the presence of the disease, but also to observe its development, and in some cases successfully remove the affected tissue surgically.

‘Another field of application of fluorophores is pharmacy: they can be used to trace how a drug (for example, an anti-cancer drug) is absorbed by various organs of the human body, what changes in human organs and tissues occur, how benign they are,’ Zyryanov adds.

The “behavior” of dyes is an indicator of the presence of explosives. In this case, the receptors of the brightly luminous fluorophore do not “touch”, as in the case of the cancer cell, but “smell” the explosive molecule in the air in the form of vapors or nanoparticles, approximately the same way a dog senses explosives with the olfactory receptors of its nose. When in contact with explosives, the fluorophore, as a rule, goes out, as the fluorescent quenching happens, which is indicated with a special device.

Fluorophores are also effective in the express analysis of soils, groundwater and domestic sewage – when it is necessary to check them for the presence of herbicides, industrial wastes or, for example, traces of pharmaceuticals. Instruments commonly used for this purpose (expensive mass spectrometers and mass chromatography mass spectrometers) are large, heavy, and require additional maintenance personnel. In contrast, to conduct an on-site visit with dyes only a small portable laboratory is required.

The uniqueness of the Ekaterinburg scientists’ technology lies in its simplicity, relative quickness and locality (due to the simultaneous passage through several stages of synthesis in one reactor). In addition, the creation of dyes is accomplished without solvents, which ensures the environmental sustainability of the process. Using the methods of “green chemistry”, waste-free and low-waste technologies is the historically established approach at the Ural Federal University. The work is carried out at the Ural Federal University’s Department of Organic and Biomolecular Chemistry and at the Ural Branch of the Russian Academy of Sciences’ Institute of Organic Synthesis under the auspices of academicians Oleg Chupakhin and Valery Charushin.

It is also important that UrFU scientists “work up” fluorophores using long-wave radiation: it is more similar to sunlight in terms of range and has great penetrating power, while affecting only the dyes and, unlike short-wave radiation, does not damage surrounding tissues, thus not doing harm to the body by secondary effects caused by radiation, such as photodegradation.

Another advantage of the technology is its low cost. Thus, it is available to medical institutions (like cancer centers) not only in large cities. Moreover, since a slight modification of the fluorophore can drastically change its properties and purpose (the same substance is suitable for working with biological objects, explosives, and pharmaceuticals), the simplicity and low cost of the technology by the Ural scientists create conditions for the synthesis of extensive “libraries” consisting of several dozen dyes for different purposes.

Clinical trials of synthesized fluorophores (the study of the aspects and limits of applicability of the synthesized dyes, the accumulation of statistics) are carried out in medical institutions and research institutes of Ekaterinburg and the Sverdlovsk region. Foreign colleagues from Europe, Brazil, India, and China also show interest in the research. Professor Ashel Silvan, a member of the editorial board of Dyes and Pigments journal, a French chemist, who recently visited the center of pharmaceutical and chemical technologies at Ural Federal University, where Professor Zyryanov’s research team works, expressed admiration for the quality of the laboratory base, scope and level of work. The Ural School of Organic Chemists, founded many years ago by the renowned academician Isaac Postovsky, confirms its leading position in global science.

Source:

https://urfu.ru/en/news/25806/

About author

Related Articles